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Sequence Analysis of TK Gene of Porcine Pseudorabies Virus Isolated from Shanxi Area
- FAN Zhen-hua, MENG Fan, WU Xin, LIU Wen-jun, YAO Jing-ming, WANG Juan-ping, HAN Yi-chao, MI Rui-juan, LEI Yu-ping
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2014, 41(4):
12-17.
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Abstract
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188 )
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TK genes of two pseudorabies virus (PRV) strains that had been isolated from Shanxi province were amplified by RT-PCR among 2009 and 2011, cloned and sequenced. The obtained sequences and the deduced amino acid were analyzed and compared with the domestic and international major epidemic strains of PRV Bartha strain, Becker strain, Ea strain, Kaplan strain, LA strain, Min-A strain, NIA-3 strain, SH strain, SL strain and Yangsan strain by homologous analysis. Sequence analysis showed that the nucleotide homologies of TK genes were 99.7%, 99.6%, 99.8%, 99.7%, 99.7%, 94.4%, 99.1%, 57.2%, 99.5% and 99.7%, respectively, and the amino acid homologies were 99.1%, 99.1%, 99.7%, 99.4%, 99.4%, 90.3%, 98.1%, 49.7%, 98.8% and 99.1%. 3 sections of GC box like sequence upstream of the initiation codon and poly adenosine polyadenylation signal AATAAA downstream of the stop codon were found in nucleotide sequence, TK gene was composed of 320 amino acids,with a conserved sequence of TK gene of herpesvirus common amino acid sequence,namely -R*Y*DG**G*GK*T- and -FDRHP*A***C*P*AR-.