鸡干扰素-α基因原核表达载体的构建及高效表达

Abstract

Abstract: Interferon (IFN)-α had received much more attention for its comprehensive antiviral activity. According to the relevant sequence from GenBank, a pair of specific primers was designed for amplifying chicken IFN-α gene with PCR method. The IFN-α gene was cloned into prokaryotic expression vector pET-32a and pET-30a-DsbA and the protein’s expression was identified by SDS-PAGE. Immunity characteristic of the protein was analyzed by Western blotting. The cloned pET-30a-DsbA-IFN-α/E.coli was found to produce a 40.4 ku protein at the high level. The expression products existed mainly in the form of inclusion body. These expression products could specificly react with His-tag monoclonal antibody.

Key words: chicken; interferon-α; prokaryotic expression

LIU Cheng-qian, WANG Jing, LI Hong, YU Zong-xing, YI Jian-zhong, CHEN Lei. Construction and High Level Expression of Prokaryotic Expression Vector of Chicken Interferon-α Gene[J]. .

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Construction and High Level Expression of Prokaryotic Expression Vector of Chicken Interferon-α Gene

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