基于转录组测序筛选调控黄羽肉鸡肌肉发育的关键基因

doi:10.16431/j.cnki.1671-7236.2025.03.002

Abstract

Abstract: 【Objective】 To improve the muscle development of Yellow-feathered chickens,RNA-Seq was used to identify key genes involved in the development of the breast muscle in fast-growing and slow-growing Yellow-feathered chickens followed by functional analysis. 【Method】 The live weight,breast muscle weight and leg muscle weight of 15-week-old Taihang chickens (slow Yellow-feathered chickens) and New Pudong chickens (fast Yellow-feathered chickens) were measured.Additionally,the breast muscle index and leg muscle index were calculated.HE stained sections were made for breast muscle and leg muscle tissues of Taihang and New Pudong chickens to analyze the histomorphological differences between two breeds.The breast muscle tissues of Taihang and New Pudong chickens were utilized as test samples for RNA-Seq to identify the differentially expressed genes (DEGs) associated with the muscle development of Yellow-feathered chickens,and 7 DEGs were randomly selected for Real-time quantitative PCR verification.【Result】 The live weight,breast muscle weight,leg muscle weight,breast muscle index and leg muscle index of Taihang chickens were extremely significantly lower than those of New Pudong chickens (P＜0.01).The muscle fiber cross sectional area and muscle fiber diameter of breast muscle in Taihang chickens were extremely significantly or significantly lower than those in New Pudong chickens (P＜0.01 or P＜0.05),and its muscle fiber density was significantly higher than that in New Pudong chickens (P＜0.05).There was no significant difference in the histomorphology of leg muscle between Taihang and New Pudong chickens (P＞0.05).A total of 332 DEGs in breast muscle between Taihang and New Pudong chickens were obtained,including 75 up-regulated genes and 257 down-regulated genes.GO function enrichment analysis showed that DEGs were mainly involved in positive regulation of smooth muscle cell migration,collagen-containing extracellular matrix and laminin binding.KEGG pathway enrichment analysis showed that DEGs were mainly enriched in focal adhesion,ECM-receptor interaction and MAPK signaling pathway.Through GO function and KEGG pathway enrichment analysis,THBL1,ENSGALG0000000814,LAMA1 and PDGFRB genes were closely related to breast muscle development.Real-time quantitative PCR results of 7 DEGs randomly selected were consistent with the expression trend of RNA-Seq,indicating that the sequencing results were reliable.【Conclusion】 THBL1,ENSGALG0000000814,LAMA1 and PDGFRB were selected as important candidate genes for breast muscle development,which laid a foundation for further analysis of muscle development of Yellow-feathered chickens.

Key words: Yellow-feathered chickens; breast muscle; growth and development; transcriptome; differentially expressed genes

CLC Number:

S831.2

WU Min, XU Junjie, LI Xinxin, CHEN Yifan, WANG Dehe, HAO Erying, CHEN Hui, SHI Lei. Screening of Key Genes Regulating Muscle Development in Yellow-feathered Chickens Based on RNA-Seq[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(3): 990-1000.

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Screening of Key Genes Regulating Muscle Development in Yellow-feathered Chickens Based on RNA-Seq

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