基于转录组和基因组数据筛选剑白香猪两头乌毛色的候选基因

doi:10.16431/j.cnki.1671-7236.2025.01.002

Abstract

Abstract: 【Objective】 The coat color phenotype of Jianhe White Xiang pigs is mostly two-end black,but the candidate genes affecting its coat color phenotype have not been reported.This study was aimed to screen the candidate genes affecting the two-end black coat color of Jianhe White Xiang pigs. 【Method】 Based on the transcriptome data of black and white skin tissues of Jianhe White Xiang pigs,differentially expressed gene screening was performed,and at the same time,the selective signals were analyzed using the data from 138 Jianhe White Xiang pigs and 50K chip of 130 Beijing Black pigs (Porcine SNP50 BeadChip),and the candidate genes affecting coat color were screened by GO function and KEGG pathway analysis. 【Result】 The results showed that 554 genes were differentially expressed in black and white coat color skin tissues in the transcriptome analysis,of which 295 were up-regulated and 259 were down-regulated.A total of 6 GO terms and 2 KEGG pathways,and a total of 12 candidate genes,such as TYR,TYRP1,DCT and PLCB4,related to the formation of hair color were screened.Based on the genome data,Fst analysis was performed,and a total of 3 GO terms and 4 KEGG pathways were screened,32 other candidate genes,such as PLCB4,FZD8 and WNT2,might be involved in the process of hair color formation in Jianhe White Xiang pigs. 【Conclusion】 Through comprehensive selection signal and transcriptome differential analysis,PLCB4 gene was identified as an important candidate gene that affected the phenotype of two-end black coat color in Jianhe White Xiang pigs.The results provided a reference for future molecular regulatory mechanisms related to coat color formation in Jianhe White Xiang pigs.

Key words: Jianhe White Xiang pigs; transcriptome; genome; coat color; candidate genes

CLC Number:

S813.3

HU Ziping, SU Yanfang, ZONG Wencheng, NIU Naiqi, ZHNAG Longchao, WANG Yuan. Screening of Candidate Genes for Two-end Black Coat Color in Jianhe White Xiang Pigs Based on Transcriptome and Genome Data[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(1): 13-24.

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Screening of Candidate Genes for Two-end Black Coat Color in Jianhe White Xiang Pigs Based on Transcriptome and Genome Data

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