1株ST515型多重耐药单增李斯特菌全基因组测序分析

doi:10.16431/j.cnki.1671-7236.2024.06.044

Abstract

Abstract: 【Objective】 The purpose of this study was to understand the drug resistance gene,virulence gene,mobile element and genetic evolution relationship of a strain of Listeria monocytogenes (LM) isolated from prepared meat products.【Method】 The resistance of LM strain LM5567 isolated from prepared meat products in Xinjiang was detected by VITEK-2 Compact and AST-GP67 susceptibility cards.After the whole genome sequencing,the functional annotation,molecular typing,genetic evolution relationship,drug resistance gene,virulence gene and mobile genetic elements of LM5567 strain were analyzed.【Result】 LM5567 strain was resistant to benzicillin,ampicillin,oxacillin,furantoin,cotrimoxazole,tetracycline and other antimicrobials.The total length of the genome was 2.974 Mb and contained 2 941 coding genes.A total of 2 144 genes were obtained by GO annotation,including 50 functional properties.Evolutionary analysis of MLST showed that the LM5567 strain was ST515 and was closely related to CC1 strain isolated from different countries.SNP analysis showed that LM5567 had the fewest mutation sites with Canadian strains 02_17924b and 02_1103, and one strain 220 from Poland.The LM5567 strain carried 88 virulence genes,6 resistance genes and 1 transposon Tn6009,which carring the tetracycline resistance gene tetM.【Conclusion】 The food-borne strain LM5567 was a multidrug resistant strain of ST515 type,carrying multiple drug resistance genes,virulence genes and 1 transposon in the genome,which had the potential for drug resistance transfer and a high risk of human listeriosis via the food chain.

Key words: Listeria monocytogenes; whole genome sequencing; virulence gene; drug resistance gene

CLC Number:

S852.61

DU Dongdong, QIAN Jing, CHANG Hengrui, LI Hongmin, WEI Xiangli, LIU Changyong, LUO Ruifeng, WANG Guohong, KANG Lichao. Whole Genome Sequencing Analysis of a ST515 Strain of Multidrug-resistant Listeria monocytogenes[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(6): 2707-2716.

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Whole Genome Sequencing Analysis of a ST515 Strain of Multidrug-resistant Listeria monocytogenes

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