山东省禽源产超广谱β-内酰胺酶大肠杆菌的分子流行病学分析

doi:10.16431/j.cnki.1671-7236.2024.01.041

Abstract

Abstract: 【Objective】 The experiment aims to analyze the drug resistance, the drug resistance mechanism, multilocus sequence typing(MLST), the virulence genes, the affinity and Inc-type plasmids of extended-spectrum β-lactamases (ESBLs) producing Escherichia coli of avian origin in Shandong province, so as to provide reference for the rational use of antibiotics in avian clinical prevention and treatment of such bacterial diseases.【Method】 38 strains of ESBLs producing E.coli, isolated from avian farms in Shandong during April 2021 to July 2021, were recovered.The antimicrobial susceptibility test of these isolates against 16 kinds of antimicrobials was determined by the broth microdilution method.The drug resistance, the virulence genes, MLST and Inc-type plasmids were detected by whole genome sequencing.Parsnp was used to analyze the phylogenetic relationship among these isolates.【Result】 The antimicrobial susceptibility test revealed that all strains were resistant to ampicillin, ceftiofur and ceftazidime.The resistance to sulfisoxazole (97.4%), trimethoprim/sulfamethoxazole (94.7%), tetracycline (89.5%), florfenicol (86.8%), spectinomycin (84.2%) was serious. Analysis results of whole genome sequencing showed that 34.2% of 38 strains of ESBLs producing E.coli carried bla_CTX-M-55 in bla_CTX-M type, 22.1% carried bla_NDM-5 and 10.5% carried bla_NDM-4 in bla_NDM type.ESBLs producing E.coli isolates carried a large number of resistance genes that mediate commonly used antimicrobials in clinical practice, and adhesion, invasion, serum resistance, iron transport virulence genes.MLST results revealed that ST10 (13.2%) was the most prevalent ST among ESBLs producing E.coli isolates, followed by ST616 (10.5%) and ST746 (10.5%).Phylogenetic tree analysis showed that ST10, ST616 and ST746 had the same origin, respectively.Inc-type plasmids results revealed that the carrier rate of IncFIB (AP001918) was the highest (68.4%), followed by IncHI2 (42.1%).【Conclusion】 The situation of ESBLs producing E.coli isolates remains very serious, bla_CTX-M mainly accounted for the resistance to extended-spectrum β-lactams antimicrobials.Moreover, this type of strain carried a large number of virulence genes, and it was urgent to strengthen monitoring.

Key words: extended-spectrum β-lactamases; carbapenem; avian Escherichia coli; whole genome sequencing; multi-locus sequence typing

CLC Number:

S852.61⁺2

SONG Xiangbin, ZHAO Xiaoyu, LI Chuanpu, MEN Xiaodong, LIANG Meng, WEI Xiuli, LI Youzhi, YANG Zhikun, ZHANG Dechen, GUO Yuqiu, TANG Wenli. Molecular Epidemiological Analysis of Avian Extended Spectrum β-lactamases Producing Escherichia coli in Shandong Province[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 407-416.

References

[1] 彭桂杰.畜禽大肠杆菌病的病症种类与防治[J].饲料博览, 2018, 11:80. PENG G J.Types and prevention of E.coli disease in livestock and poultry[J].Feed Review, 2018, 11:80.(in Chinese)
[2] ALLOCATI N, MASULLI M, ALEXEYEV M F, et al.Escherichia coli in Europe:An overview[J].International Journal of Environmental Research and Public Health, 2013, 10(12):6235-6254.
[3] 吴翠蓉, 黄璐璐, 黄俊红, 等.我国猪、鸡源沙门菌和大肠埃希菌的耐药研究进展[J].中国抗生素杂志, 2021, 46(6):509-517. WU C R, HUANG L L, HUANG J H, et al.Research progress on antimicrobial resistance of pig- and chicken-derived Salmonella and Escherichia coli in China[J].Chinese Journal of Antibiotics, 2021, 46(6):509-517.(in Chinese)
[4] 赵效南, 张印, 胡明, 等.山东省肉鸡养殖场大肠杆菌的分离鉴定及抗药性监测[J].山东农业科学, 2021, 53(2):123-126. ZHAO X N, ZHANG Y, HU M, et al.Isolation, identification and drug resistance surveillance of Escherichia coli strains from broiler farms in Shandong province[J].Shandong Agricultural Sciences, 2021, 53(2):123-126.(in Chinese)
[5] 唐标, 陈怡飞, 陈聪, 等.2019年湖南省部分地区鸡、鸭、猪源大肠杆菌耐药性调查[J].畜牧与兽医, 2021, 53(4):54-60. TANG B, CHEN Y F, CHEN C, et al.Investigation of antimicrobial resistance of Escherichia coli from chickens, ducks and pigs in some areas of Hunan province in 2019[J].Animal Husbandry & Veterinary Medicine, 2021, 53(4):54-60.(in Chinese)
[6] POIRE L, MADEC J Y, LUPO A, et al.Antimicrobial resistance in Escherichia coli[J].Microbiology Spectrum, 2018, 6(4):ARBA-0026-2017.
[7] 刘保光, 栗俞程, 汪保英, 等.CTX-M型超广谱β-内酰胺酶研究进展[J].河南农业科学, 2019, 48(12):1-7. LIU B G, LI Y C, WANG B Y, et al.Research progress of CTX-M type extended-spectrum β-lactamases[J].Henan Agricultural Sciences, 2019, 48(12):1-7.(in Chinese)
[8] 汤文利, 郭玉秋, 门晓冬, 等.山东省畜禽源大肠杆菌的分离鉴定和药物敏感性调查[J].中国兽医杂志, 2023, 59(5):75-79. TANG W L, GUO Y Q, MEN X D, et al.Isolation, identification and antimicrobial drug sensitivity surveillance of Escherichia coli from livestock and poultry in Shandong province[J].Chinese Journal of Veterinary Medicine, 2023, 59(5):75-79.(in Chinese)
[9] CLINICAL AND LABORATORY STANDARDS INSTITUTE.Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals.Second informational supplement.CLSI document VET01-S2[S].2013.
[10] CLINICAL AND LABORATORY STANDARDS INSTITUTE.Performance standards for antimicrobial susceptibility testing.Twenty-eighth informational supplement.CLSI document M100-S28[S].2018.
[11] 何涛.动物源大肠杆菌对乙酰甲喹和氟苯尼考的敏感性折点及分子耐药机制研究[D].北京:中国农业大学, 2015. HE T.Study on the susceptible breakpoint and molecular resistance mechanism for mequindox and florfenicol against animal-derived E.coli[D].Beijing:China Agricultural University, 2015.(in Chinese)
[12] 李佳瑞.安普霉素对猪源大肠杆菌野生型折点及其耐药机制研究[D].哈尔滨:东北农业大学, 2019. LI J R.Wild-type cutoff and resistance mechanism of apramycin against swine-derived Escherichia coli[D].Harbin:Northeast Agricultural University, 2019.(in Chinese)
[13] NANG S C, AZAD M A K, VELKOV T, et al.Rescuing the last-line polymyxins:Achievements and challenges[J].Pharmacological Reviews, 2021, 73(2):679-728.
[14] 郑红青, 邓玉婷, 何良英, 等.食品动物源产超广谱β-内酰胺酶大肠杆菌的流行状况调查[J].中国畜牧兽医, 2011, 38(12):163-166. ZHENG H Q, DENG Y T, HE L Y, et al.Investigation of the prevalence of Escherichia coli from ultra-broad-spectrum β-lactamase of food animal origin[J].China Animal Husbandry & Veterinary Medicine, 2011, 38(12):163-166.(in Chinese)
[15] NORDMANN P, POIREL L, WALSH T R, et al.The emerging NDM carbapenemases[J].Trends in Microbiology, 2011, 19(12):588-595.
[16] 刘文双, 魏秀丽, 赵云亭, 等.山东地区蛋鸡源大肠杆菌bla_CTX-M型超广谱β-内酰胺酶耐药基因的流行传播研究[J].山东农业科学, 2022, 54(9):142-147. LIU W S, WEI X L, ZHAO Y T, et al.Epidemic transmission of bla_CTX-Mextended-spectrum β-lactamase resistance gene in Escherichia coli derived from laying hens in Shandong region[J].Shandong Agricultural Sciences, 2022, 54(9):142-147.(in Chinese)
[17] 杜向党, 焦显芹, 莫娟, 等.鸡猪源大肠杆菌CTX-M型ESBLs的分子检测[J].华北农学报, 2009, 24(2):90-93. DU X D, JIAO X Q, MO J, et al.Molecular detection of CTX-M type ESBLs genes among Escherichia coli strains isolated from diseased chickens and pigs[J].Acta Agriculturae Boreali-Sinica, 2009, 24(2):90-93.(in Chinese)
[18] 赵凤菊, 曹东, 李井春, 等.猪源大肠埃希菌超广谱β-内酰胺酶的检测及其耐药性分析[J].河南农业科学, 2017, 46(2):111-115. ZHAO F J, CAO D, LI J C, et al.Detection and analysis of drug resistance of ESBLs in Escherichia coli strains isolated from swine[J].Journal of Henan Agricultural Sciences, 2017, 46(2):111-115.(in Chinese)
[19] 孙乃岩, 龚倩梅, 胡建春, 等.屠宰场鸡源碳青霉烯耐药大肠埃希菌的耐药特征分析[J].南京农业大学学报, 2022, 45(6):1203-1210. SUN N Y, GONG Q M, HU J C, et al.Antimicrobial resistance characteristics of carbapenem-resistance Escherichia coli from chickens in slaughterhouse[J].Journal of Nanjing Agricultural University, 2022, 45(6):1203-1210.(in Chinese)
[20] 李翠函, 曲志娜, 高玉斌, 等.三类禽源碳青霉烯类耐药大肠杆菌流行病学调查及全基因组测序分析[J].中国畜牧兽医, 2023, 50(4):1653-1662. LI C H, QU Z N, GAO Y B, et al.Epidemiology investigation and whole genome sequencing analysis of three types of poultry originated carbapenem-resistant Escherichia coli[J].China Animal Husbandry & Veterinary Medicine, 2023, 50(4):1653-1662.(in Chinese)
[21] 马振报, 庞虹, 许娃丽, 等.广东地区黄羽肉鸡肠杆菌中碳青霉烯耐药基因的流行病学调查[J].中国兽医杂志, 2023, 59(4):19-26. MA Z B, PANG H, XU W L, et al.Epidemiological investigation of carbapenem-resistant Enterobacteriaceae from Yellow-feathered broiler in Guangdong province[J].Chinese Journal of Veterinary Medicine, 2023, 59(4):19-26.(in Chinese)
[22] 裴建武, 甘孟侯.鸡致病性大肠杆菌研究进展[J].中国兽医学报, 1997, 17(2):104-105. PEI J W, GAN M H.Research progress on pathogenic Escherichia coli in chickens[J].Chinese Journal of Veterinary Science, 1997, 17(2):104-105.(in Chinese)
[23] 杨文文, 李玉保, 路建彪, 等.鸡源大肠杆菌携带毒力基因、血清型及与致病性相关性的研究[J].中国预防兽医学报, 2021, 43(10):1051-1056. YANG W W, LI Y B, LU J B, et al.Study on the correlation among virulence genes number, serotype and the pathogenicity of chicken E.coli[J].Chinese Journal of Preventive Veterinary Medicine, 2021, 43(10):1051-1056.(in Chinese)
[24] FU D, WU J, GU Y, et al.The response regulator OmpR contributes to the pathogenicity of avian pathogenic Escherichia coli[J]. Poultry Science, 2022, 101(4):101757.
[25] JAYALAXMI W, DEBADATTA D C, AMITABHA B, et al.Expression of itaT toxin gene is enhanced under aminoglycoside stress in Escherichia coli harbouring aac(6')-Ⅰb[J].Gene Reports, 2022, 26:101526.
[26] PEIRANO G, CHEN L, N OBREGA D, et al.Genomic epidemiology of global carbapenemase-producing Escherichia coli, 2015-2017[J].Emerging Infectious Diseases, 2022, 28(5):924-931.
[27] ZHOU W, LIN R, ZHOU Z, et al.Antimicrobial resistance and genomic characterization of Escherichia coli from pigs and chickens in Zhejiang, China[J].Frontiers in Microbiology, 2022, 13:1018682.
[28] 王可.动物源产ESBL大肠杆菌的分离鉴定与耐药特性研究[D].乌鲁木齐:新疆农业大学, 2020. WANG K.Isolation, identification and drug resistance characteristics of ESBL-producing E coli from animal sources[D].Urumqi:Xinjiang Agricultural University, 2020.(in Chinese)
[29] ZENG Z, YANG J, GU J, et al.Prevalence and antimicrobial susceptibility of CTX-M-type-producing Escherichia coli from a wildlife zoo in China[J].Veterinary Medicine and Science, 2022, 8(3):1294-1299.
[30] CARATTOLI A.Resistance plasmid families in Enterobacteriaceae[J].Antimicrobial Agents and Chemotherapy, 2009, 53(6):2227-2238.
[31] MEDINA-PIZZALI M L, VENKATESH A, RIVEROS M, et al.Whole-genome characterisation of ESBL-producing E.coli isolated from drinking water and dog faeces from rural andean households in Peru[J].Antibiotics (Basel), 2022, 11(5):692.
[32] SHAFIQ M, RAHMAN S U, BILAL H, et al.Incidence and molecular characterization of ESBL-producing and colistin-resistant Escherichia coli isolates recovered from healthy food-producing animals in Pakistan[J].Journal of Applied Microbiology, 2022, 133(3):1169-1182.

Molecular Epidemiological Analysis of Avian Extended Spectrum β-lactamases Producing Escherichia coli in Shandong Province

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 10

Recommended Articles

Metrics

Comments

[1]	LI Cuihan, QU Zhina, GAO Yubin, LI Yan, WANG Juan, DUAN Xiaoxiao, WANG Lin, ZHANG Xiyue, ZHAO Ge, HUANG Xiumei, ZHAO Jianmei, ZHANG Qingqing, WANG Junwei, HUANG Baoxu, LIU Junhui. Epidemiology Investigation and Whole Genome Sequencing Analysis of Three Types of Poultry Originated Carbapenem-resistant Escherichia coli [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1653-1662.
[2]	LIU Kexin, WANG Chao, ZHANG Ao, LIU Jiali, TAN Bin, ZHANG Shuqin. Isolation,Identification and Genome Sequence Analysis of Bovine Viral Diarrhea Virus LN-1 Strain [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1150-1159.
[3]	GAO Duo, WANG Jinyu, LI Heyu, JIANG Qifeng, CHEN Zihan, XU Xiying, JIA Kun. Isolation,Identification and Biological Characterization Analysis of a Bacteriophage GD01 from Salmonella Enteritidis [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(12): 5160-5169.
[4]	LI Jinmin, GAO Xuna, SANG Ruixin, GONG Benzhi, XU Haiyan, GU Wei, HAO Muqiang, LAN Jianghua, WANG Hong. Isolation and Evaluation of Sterilization Effect of an Avian Escherichia coli Phage [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 783-190.
[5]	XU Minsheng, KE Haiyi, YANG Dongxia, SHI Keda, SUN Zeyi, NIU Jiawei, CHANG Xin, ZHAI Shaolun, ZANG Yingan, LI Chunling. Whole Genome Sequencing and Biological Characteristics Analysis of Multidrug-resistant Actinobacillus pleuropneumoniae GD2107 Strain [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 4019-4031.
[6]	YANG Dezhi, HOU Guanyu, SHI Liguang, CAO Ting, ZHOU Xiong, ZHAO Yanhong. Research Progress on Whole Genome Sequencing in Livestock and Poultry [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3403-3414.
[7]	WU Shuaibin, CUI Zehua, LIAO Xiaoping, LIU Yahong, SUN Jian. Research Progress on the Detection Methods of Carbapenemase-producing Enterobacteriaceae [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 1072-1083.
[8]	LUAN Yongjiao, XIE Zhixun, WANG Sheng, LUO Sisi, ZHANG Lei, XIE Liji, XIE Zhiqin, DENG Xianwen, ZHANG Minxiu, ZHANG Yanfang, ZENG Tingting, FAN Qing. Whole-genome Sequencing and Analysis of Fowl Adenovirus Serotype 4 Strain GX2019-010 Isolated from Guangxi [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 3793-3804.
[9]	ZHANG Jingju, LIU Jing, XU Fei. Research Progress on Resistance of Salmonella Kentucky to Fluoroquinolones and Carbapenems [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(11): 3739-3748.
[10]	SHI Ke;JIA Yan-hua;WU Hua;LI Sheng-li;YANG Liang;HU Gong-zheng. Selection of Chinese Medicine which has Inhibitory Action Against Extended-Spectrum β-Lactamases [J]. , 2008, 1(3): 83-85.