1株禽大肠杆菌噬菌体的分离及杀菌效果评估

doi:10.16431/j.cnki.1671-7236.2022.02.041

Abstract

Abstract: 【Objective】 The study was aimed to explore a new way to control environmental pathogenic Escherichia coli (E.coli).【Method】 The isolation and corresponding index evaluation of Escherichia coli phages were carried out in this study.The lytic phages of avian pathogenic E.coli were isolated from breeding environment by double-layer plate method.The phage was comprehensively evaluated through electron microscopy and enzyme digestion identification, stability, optimal multiplicity of infection, one-step growth curve and its bactericidal effect in simulated environment.【Result】 The isolated phage had a regular polyhedron head and a slender tail structure, with a head diameter of about 98 nm and a tail length of about 123 nm.Combined with the results of enzyme digestion, it was preliminarily identified as a double-stranded DNA phage of Cauda muscularis.Its temperature tolerance range was 37-50 ℃, pH tolerance range was 3.0-11.0.When the multiplicity of infection was 0.00001, the titer of progeny phage was the highest.One-step growth curve showed that the incubation period of the phage was about 20-40 min, the lysis time was about 80-100 min, and the lysis amount was 4 133 PFU/cell.It could be seen from the bactericidal effect of the phage on the host E.coli in the simulated environment that the bactericidal rate of phage ФECP2-1 with the concentration of 1×10⁴-1×10⁶ PFU/mL against the target E.coli in the liquid for 1-6 h was above 99.9%.The phage ФECP2-1 with the concentration of 2×10⁴-2×10⁶ PFU/g could kill more than 99% of the target E.coli in chicken manure for 5-10 h.The phage ФECP2-1 had a slightly lower bactericidal effect on the target E.coli in chicken manure than on the target E.coli in liquid.【Conclusion】 ФECP2-1 conformed to the characteristics of phage disinfectants, and was a phage with good application prospect.It could be used as a biological disinfectant for the prevention and control of avian E.coli in aquaculture environment.

Key words: avian Escherichia coli; pyrolytic phages; biological characteristics; sterilization effect

CLC Number:

S852.65

LI Jinmin, GAO Xuna, SANG Ruixin, GONG Benzhi, XU Haiyan, GU Wei, HAO Muqiang, LAN Jianghua, WANG Hong. Isolation and Evaluation of Sterilization Effect of an Avian Escherichia coli Phage[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 783-190.

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Isolation and Evaluation of Sterilization Effect of an Avian Escherichia coli Phage

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