1株鲤鱼源植物乳杆菌的分离鉴定及其生物学特性分析

doi:10.16431/j.cnki.1671-7236.2022.07.039

Abstract

Abstract: 【Objective】 The aim of this study was to screen the cyprin-derived lactic acid bacteria with excellent probiotics as candidate bacterial strains for aquaculture probiotics.【Method】 In this experiment,the intestinal contents and mucous membrane of carp were used as the sources of bacterial isolates.MRS medium was used for bacterial isolates and purification.After identification by 16S rRNA sequencing,the biological characteristics of isolated strains,such as growth characteristics,acid production capacity,acid tolerance,bile salt tolerance,antibacterial characteristics,drug sensitivity and safety were analyzed.【Result】 The results showed that a strain of Lactobacillus plantarum was successfully isolated and named YY001.On MRS medium,the colony morphology of the isolated strain was round,smooth surface,neat edge,milky white,Gram-positive,spun-free,blunt short-bacillus.The strain began to grow in logarithmic phase after 4 h,and reached a stable phase after 12 h.The strain had a strong acid production capacity.The acid production curve showed that pH decreased rapidly from 0 to 12 h,and pH reached 3.8 after 16 h culture.The strain had a certain tolerance to acid and bile salt at pH 3.0 and 0.3% bile salt.The strain had bacteriostatic effect on Flavobacterium columnar,Aeromonas vibrii and Aeromonas hydrophila,and the bacteriostatic effect on Flavobacterium columnar was very significant,and the diameter of bacteriostatic zone was 34.19 mm.The drug sensitivity test showed that the strain was resistant to kanamycin,streptomycin and vancomycin.Once a day for one week,10⁸ CFU isolated strain had no toxic effect on mice after feeding.【Conclusion】 The strain YY001 isolated in this study had excellent biological characteristics,which could be used as a candidate strain for aquaculture probiotics,and laid a foundation for the subsequent preparation of probiotics for carp.

Key words: carp; Lactobacillus plantarum; isolation and identification; biological characteristics

CLC Number:

S816.73

YANG Zemin, LI Shuang, JIN Zhengyu, LIAO Yixiao, YANG Ying, WEN Ming. Isolation,Identification and Biological Characteristics of a Strain of Lactobacillus plantarum from Carp[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2805-2811.

References

[1] WANKA K M,DAMERAU T,COSTAS B,et al.Isolation and characterization of native probiotics for fish farming[J].Bmc Microbiology,2018,18(1):119.
[2] MARTINEZ CRUZ P,IBANEZ A L,MONROY HERMOSILLO O A,et al.Use of probiotics in aquaculture[J].ISRN Microbiology,2012,20(12):916845.
[3] HAI N V.The use of probiotics in aquaculture[J].Journal of Applied Microbiology,2015,119(4):917-935.
[4] 尹珺伊,刘秋瑾,田秋丰,等.一株干酪乳杆菌的分离鉴定及生物学特性研究[J].中国畜牧兽医,2021,48(2):516-524. YI J Y,LIU Q J,TIAN Q F,et al.Isolation,identification and biological characteristics of a strain of Lactobacillus casei[J]. China Animal Husbandry & Veterinary Medicine,2021,48(2):516-524.(in Chinese)
[5] 孙磊,魏宇超,陈宝江.3种益生菌在家兔生产中应用研究进展[J].中国养兔,2020,5:17-19. SUN L,WEI Y C,CHEN B J.Application of three kinds of probiotics in rabbit[J].Chinese Journal of Rabbit Farming,2020,5:17-19.(in Chinese)
[6] 杜艺.丁酸梭菌和丁酸钠对鲤鱼生长和肠道免疫的影响[D].南京:南京农业大学,2019. DU Y.Effects of Clostridium butyricum and sodium butyrate on growth and intestinal immunity of common carp[D].Nanjing:Nanjing Agricultural University,2019.(in Chinese)
[7] 牛春宇,石琳琳,孙苗,等.组学技术在乳酸菌分离鉴定及功能研究中的应用[J].中国畜牧兽医,2020,47(12):3882-3889. NIU C Y,SHI L L,SUN M,et al.Application of omics appreaches in isolation and identification of lactic acid bacteria and it's functional research[J].China Animal Husbandry & Veterinary Medicine,2020,47(12):3882-3889.(in Chinese)
[8] 吴重德,黄钧,周荣清.调控乳酸菌酸胁迫抗性研究进展[J].微生物学报,2014,54(7):721-727. WU C,HUANG J,ZHOU R.Regulating acid stress resistance of lactic acid bacteria--A review[J].Acta Microbiologica Sinica,2014,54(7):721-727.(in Chinese)
[9] 李利,李泽健.饲粮中添加乳酸菌对鲤鱼生长性能、鱼体组成及肠道酶活性的影响[J].中国饲料,2021,13:80-83. LI L,LI Z J.Effects of dietary lactic supplementation on growth performance,body composition and intestinal enzyme activity of common carp[J].China Feed,2021,13:80-83.(in Chinese)
[10] 桂远明,何幽峰,吴垠,等.生态制品(Jy10、Jy31复合制剂)饲料添加剂对提高鲤抗病力的研究[J].中国微生态学杂志,1994,6:27-33. GUI Y M,HE Y F,WU Y,et al.Study on improvement of disease resistance of Cyprinus carpio by feed additive of ecological products (Jy10,Jy31 compound preparation)[J].Chinese Journal of Microecology,1994,6:27-33.(in Chinese)
[11] 何宇智.使用微生态制剂和玉米糖渣对草鱼养殖中相关指标的影响[D].广州:华南农业大学,2018. HE Y Z.Effects of probiotics and corn sugar related indicators in culture of grass carp[D].Guangzhou:South China Agricultural University,2018.(in Chinese)
[12] 陈金龙,李书光,张莎莎,等.一株猪源链球菌的分离鉴定及生物学特性研究[J].中国畜牧兽医,2012,39(7):74-77. CHEN J L,LI S G,ZHANG S S,et al.Identification and biological characteristics of a strain Streptococcus suis[J].China Animal Husbandry & Veterinary Medicine,2012,39(7):74-77.(in Chinese)
[13] NAYAK S K.Multifaceted applications of probiotic Bacillus species in aquaculture with special reference to Bacillus subtilis[J].Reviews in Aquaculture,2021,13(2):862-906.
[14] 杨运楷.复合益生菌在凡纳滨对虾养殖中的应用及评价[D].上海:上海海洋大学,2019. YANG Y K.Application and evaluation of compound probiotics in the culture of Litopenaeus vannamei[D].Shanghai:Shanghai Ocean University,2019.(in Chinese)
[15] 管婷婷.泥鳅肠道益生菌的筛选及应用研究[D].大连:大连海洋大学,2016. GUAN T T.Studies on selection of probiotics from the Misgurnus anguillicaudatus and their application[D].Dalian:Dalian Ocean University,2016.(in Chinese)
[16] 桂琳.复合益生菌对中华鳖、草鱼生长及血液指标的影响[D].长沙:湖南农业大学,2015. GUI L.Effects of compound probiotics on growth and blood index of Trionyx sinensis and Ctenopharyngodon idellus[D].Changsha:Hunan Agricultural University,2015.(in Chinese)
[17] 李军亮,杨奇慧,谭北平,等.嗜酸乳杆菌对斜带石斑鱼幼鱼生长、消化性能和抗病力的影响[J].水生生物学报,2019,43(5):992-1000. LI J L,YANG Q H,TAN B P,et al.Effects of Lactobacillus acidophilus on growth,digestive performance and disease resistance of Juvenile grouper[J].Acta Hydrobiologica Sinica,2019,43(5):992-1000.(in Chinese)
[18] MARIE KAKTCHAM P,TEMGOUA J B,ZAMBOU F N,et al.In vitro evaluation of the probiotic and safety properties of bacteriocinogenic and non-bacteriocinogenic lactic acid bacteria from the intestines of Nile tilapia and common carp for their use as probiotics in aquaculture[J].Probiotics and Antimicrobial Proteins,2018,10(1):98-109.
[19] 曾东.鲤益生菌的筛选、生物学特性及作用机理的研究[D].雅安:四川农业大学,2009. ZENG D.The study of isolaton,biological characteristics and mechanism of probiotics from Cyprinus carpio[D].Ya'an:Sichuan Agricultural University,2009.(in Chinese)
[20] BOEDELTJE G,JONGEJANS E,SPANINGS T,et al.Effect of gut passage in fish on the germination speed of aquatic and riparian plants[J].Aquatic Botany,2016,13(2):12-16.
[21] 杨悦,滑婉月,陈志迪,等.植物乳杆菌LV02抑菌特性及发酵培养基优化研究[J].福建农业学报,2021,36(1):91-103. YANG Y,HUA W Y,CHEN Z D,et al.Study on bacteriostatic characteristics of Lactobacillus plantarum LV02 and optimization of fermentation medium[J].Fujian Journal of Agricultural Sciences,2021,36(1):91-103.(in Chinese)
[22] 孔祥丽,吴昕雨,许晓曦.植物乳杆菌代谢产物抑菌机制与应用研究进展[J].食品安全质量检测学报,2021,12(8):3131-3140. KONG X L,WU X Y,XU X X.Research progress on metabolites and bacteriostasis mechanism of Lactobacillus plantarum[J].Journal of Food Safety & Qualit,2021,12(8):3131-3140.(in Chinese)
[23] 张洪玉.干酪乳杆菌YYL3和植物乳杆菌YYL5的生物学特性及其对斑点叉尾鮰生长、免疫及肠道菌群的影响[D].上海:上海海洋大学,2019. ZHANG H Y.The biologiical characteristics of Lactobacillus casei YYL3 and Lactobacillus plantarum YYL5 and their effects on growth,immune response and intestinal microbiota in channel catfish,Ictalurus punctatus[D].Shanghai:Shanghai Ocean University,2019.(in Chinese)
[24] DANIALI M,NIKFAR S,ABDOLLAHI M.Antibiotic resistance propagation through probiotics[J].Expert Opinion on Drug Metabolism & Toxicology,2020,16(12):1207-1215.

Isolation,Identification and Biological Characteristics of a Strain of Lactobacillus plantarum from Carp

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