内蒙古地区乳房炎奶样与环境中细菌的分离鉴定及耐药性分析

doi:10.16431/j.cnki.1671-7236.2023.02.039

Abstract

Abstract: 【Objective】 This study was aimed to investigate the species and drug resistance of pathogenic bacteria in raw milk samples and environmental samples of mastitis from some dairy farms in Inner Mongolia,so as to provide clinical medication guidance and reference for the prevention and control of mastitis.【Method】 Bacteria were isolated from 468 samples (199 samples of mastitis raw milk and 269 samples of mastitis cow breeding environment) from 4 large-scale farms in Inner Mongolia by plate streak method.The isolated bacteria were identified by morphological observation,Gram staining microscopy and 16S rDNA sequencing.Drug susceptibility testing was performed on the main pathogenic bacteria that cause mastitis.【Result】 There were small,white and yellow colonies on the high salt mannitol medium,56 strains were highly similar to Staphylococcus aureus by 16S rDNA sequencing and comparison,and the isolation rate was 11.97%.On eosin-methylene blue medium,there were black purple and metallic luster colonies.After 16S rDNA sequencing and comparison,44 strains were highly similar to Escherichia coli,with a separation rate of 9.40%.In 199 raw milk samples,the isolation rates of Staphylococcus aureus and Escherichia coli were the highest,it was 21.11% and 17.09%,respectively.In 269 environmental samples,the isolation rates of Staphylococcus aureus and Escherichia coli were 5.20% and 3.72%,respectively.The results of drug sensitivity test showed that Staphylococcus aureus was relatively resistant to penicillin,sulfamethoxazole,ampicillin,amoxicillin/clavulanic acid,and the drug resistance rates were 90.63%,78.13%,75.00% and 68.75%,respectively,and the sensitivity rate to gentamicin,ciprofloxacin and compound sulfamethoxazole was higher. Escherichia coli was significantly resistant to ampicillin,cefothiophene and sulfamethoxazole,and the drug resistance rates were 100%,94.29% and 45.71%,respectively,it was highly sensitive to streptomycin,kanamycin,gentamycin,polymyxin E,meropenem,amoxicillin/clavulanic acid and rifaximin.【Conclusion】 The main pathogenic bacteria causing dairy cow mastitis in Inner Mongolia were Staphylococcus aureus and Escherichia coli. Staphylococcus aureus had relatively high resistance to penicillin,sulfamethoxazole,ampicillin,amoxicillin/clavulanic acid, Escherichia coli had obvious drug resistance to ampicillin,cephalothiophene and sulfamethoxazole.

Key words: diary cow mastitis; pathogenic bacteria; isolation and identification; drug resistance; drug sensitivity test

CLC Number:

S852.6

ZHONG Huachen, WANG Lifang, GUO Chenyang, LIU Jialin, SONG Jie. Isolation,Identification and Drug Resistance Analysis of Pathogenic Bacteria from Milk Samples and Environment of Mastitis in Inner Mongolia[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 817-826.

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Isolation,Identification and Drug Resistance Analysis of Pathogenic Bacteria from Milk Samples and Environment of Mastitis in Inner Mongolia

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