Abstract: Exosomes are nanoscale and homogeneous membranous vesicles that widely exist in various body fluid environments and some tissues,which are mainly composed of various biological components such as proteins,lipids,mRNA,miRNA and lncRNA.In recent years,researchers have isolated exosomes from the follicular fluid of different animals.In addition,it has been scientifically confirmed that follicular fluid exosomes are involved in a series of reproductive processes,such as proliferation of follicular granulosa cells,egg formation and development,animal fertilization,embryo development,and male animal reproductive function,which provides new ideas for further research on the regulation of follicular fluid exosomes on mammalian reproductive function.Therefore,the author described the latest progress in the isolation and identification of exosomes from follicular fluid,their main components and the molecular mechanisms regulating mammalian reproductive function,which provided a reference for a comprehensive understanding of the structure and function of exosomes,germinal apoptosis and mechanism of action.At the same time,it provided relevant basic information for exploring animal reproductive theory and improving animal reproductive technology using exosomes and applying it to practical production.

Key words: exosomes; isolation and identification; follicular fluid