牛源抗氧化芽孢杆菌的分离鉴定及生物特性研究

doi:10.16431/j.cnki.1671-7236.2023.01.036

Abstract

Abstract: 【Objective】 This study was aimed to isolate Bacillus with antioxidant capacity and good tolerance from the rumen of dairy cows, so as to provide theoretical basis and reference for the use of bovine probiotics as antioxidant additives.【Method】 The rumen contents of 3 adult Holstein cows were collected and identified by bacterial isolation and purification, morphological observation, physiological and biochemical detection and 16S rDNA molecular detection.The scavenging rates of superoxide free radicals and hydroxyl free radicals, acid resistance, bile salt resistance and antibiotic resistance of the screened strains were also detected.【Result】 A total of 12 strains of Bacillus were isolated from rumen fluid of dairy cows, and 5 strains of Bacillus (X-1 to X-5) were screened by specific medium and Gram staining.Five strains of Bacillus were identified by molecular identification, and X-1 was identified as Bacillus thermoamylovorans, X-2 as Bacillus pumilus, X-3 as Bacillus proteolyticus, X-4 as Bacillus subtilis, and X-5 as Bacillus safensis.The results of antioxidant capacity test showed that all strains had certain antioxidant capacity, and strain X-5 had the highest antioxidant capacity, with the scavenging rates of superoxide free radicals and hydroxyl free radicals being 73.81% and 73.54%, respectively.When pH was 2.5, the survival rates of 5 Bacillus strains were all more than 25%.When the bile salt concentration was 0.6%, the survival rates of five Bacillus strains were all more than 60%, indicating that the strains had good tolerance.The results of drug sensitivity test showed that most of the isolates were highly sensitive to ofloxacin, nitrofurantoin, erythromycin and so on.It was resistant to cefuroxime, cefotaxime, streptomycin and so on.It was moderately sensitive to several drugs such as kanamycin, ciprofloxacin and ampicillin.【Conclusion】 The isolated Bacillus from cattle in this study had antioxidant capacity, and some of the strains had good acid resistance and bile salt resistance, which provided theoretical basis and reference for the use of Bacillus from cattle as antioxidant additives.

Key words: dairy cows; Bacillus; isolation and identification; oxidation resistance; biochemical characteristics

CLC Number:

S816.7

SUN Yu, ZHANG Hao, LI Changyang, TAO Yanzi, WANG Qiuju. Isolation,Identification and Biological Characteristics of Antioxidant Bacillus from Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 359-367.

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Isolation,Identification and Biological Characteristics of Antioxidant Bacillus from Cattle

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