China Animal Husbandry and Veterinary Medicine ›› 2023, Vol. 50 ›› Issue (2): 827-837.doi: 10.16431/j.cnki.1671-7236.2023.02.040

• Clinical Veterinary Medicine • Previous Articles     Next Articles

Research Progress on Optical and Electrochemical Biosensors for the Diagnosis of Important Diseases in Dairy Cows

HE Yue1,2, WANG Hui1, CHEN Ruipeng1, YU Zhixue1, TANG Xiangfang1, GUO Yuming2, XIONG Benhai1   

  1. 1. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    2. State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
  • Received:2022-09-20 Online:2023-02-05 Published:2023-02-06

Abstract: With the large-scale and intensive development of dairy farming in China,and the excessive pursuit of dairy production performance,it is easy for dairy cows to occur diseases.Subclinical ketosis,subclinical hypocalcemia,mastitis,foot-and-mouth disease,respiratory diseases and bovine viral diarrhea are mainly nutritional and infectious diseases,which threaten the health and welfare of dairy cows and bring about the decrease of milk quality and the increase of financial loss of farm.The timely and rapid detection of diseases is a key step to control the infection among livestock,reduce the damage of economy,and protect the food safety.Although cell culture methods,enzyme-linked immunoabsorbent assay (ELISA),polymerase chain reaction (PCR) and some other methods have been applied in the detection of diseases of livestock and poultry,it’s still difficult to carry on the detecting work due to the limitations of time consuming and high-cost and complicated procedures of present methods,and unsuitable for point-of-care testing of diseases of dairy cow.Biosensor depends on a transductor to convert the sign originating from specific biochemical interaction between the biomarker and the bioreceptor into a measurable signal,and on a signal amplification system to detect the biomarker qualitatively or quantitatively.There is great potential for biosensor in detecting metal ion,pathogenic bacteria with advantages of simple operation,high sensitivity and strong specificity.Currently,there are various kinds of biosensors designed for the detection of diseases of diary cow.In this review,we provided an overview of the corresponding electrochemical and optical biosensors for common diseases of dairy cows.The descriptions illustrated the detection strategy,detection range and limit and other information of each biosensor.In the last section,this paper prospected the development tendency of biosensor,in order to provide references for the healthy development of dairy cow.

Key words: biosensor; photoelectric sensing; dairy cows; disease diagnosis

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