健康奶牛生殖道乳酸菌的分离鉴定及其抑菌活性研究

doi:10.16431/j.cnki.1671-7236.2022.05.025

Abstract

Abstract: 【Objective】 It was aimed to isolate and screen probiotic lactic acid bacteria from the reproductive tract of healthy dairy cows,and to provide probiotic strains for the prevention and treatment of dairy cow endometritis in the future,and lay the foundation for the development of microecological preparations for the prevention and treatment of this disease.【Method】 The reproductive tract secretion samples of 10 healthy dairy cows were collected and isolated and cultured with MRS medium.The isolated strains were identified by morphological observation and 16S rDNA sequence.The sequence results were compared with the reference strains on NCBI nucleic acid database to determine the species of each isolated strain.Oxford cup method was used to isolate and screen strains with good antibacterial activity.The growth curve,acid production ability and antimicrobial sensitivity of these screened strains were studied,and their main antibacterial substances were detected.【Result】 A total of 13 strains of lactic acid bacteria were isolated from 10 samples.After staining microscopy and 16S rDNA sequencing analysis,13 strains of lactic acid bacteria were identified as 11 strains of Lactobacillus plantarum,1 strain of Enterococcus specialis and 1 strain of Enterococcus rodentis respectively.5 strains of lactic acid bacteria with good antibacterial ability against Escherichia coli were obtained by single strain antibacterial test.The growth characteristics and acid production ability of the five strains of lactic acid bacteria were good,and they were highly sensitive to ampicillin,tetracycline,erythromycin,rifampicin,trimethoprim,chloramphenicol and penicillin.The antibacterial substances of strains 21 and 35 were hydrogen peroxide and bacteriocin,while the antibacterial substances of strains 25,33 and 34 included organic acids in addition to hydrogen peroxide and bacteriocin.【Conclusion】 Three strains of lactic acid bacteria isolated from the reproductive tract of dairy cows were expected to be used as probiotics in the clinical prevention and treatment of dairy cow endometritis,and its specific prevention and treatment effect needed to be further explored.

Key words: dairy cows; endometritis; lactic acid bacteria; antibacterial activity

CLC Number:

S858.23

SUN Yan, FENG Xiaowei, LIU Jiawei, LIU Bei, JIN Tianxiong, FENG Mingque, LIU Mingchao. Isolation,Identification and Antibacterial Activity of Lactic Acid Bacteria from Reproductive Tract of Healthy Dairy Cows[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1852-1859.

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Isolation,Identification and Antibacterial Activity of Lactic Acid Bacteria from Reproductive Tract of Healthy Dairy Cows

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