益母草碱对LPS诱导小鼠子宫内膜炎的抗炎作用研究

doi:10.16431/j.cnki.1671-7236.2023.05.041

Abstract

Abstract: 【Objective】 The aim of this study was to investigate the anti-inflammatory effect of leonurine on lipopolysaccharide (LPS)-induced endometritis in mice.【Method】 Sixty female C57 mice aged 6 to 8 weeks were randomly divided into 6 groups:Blank control group,model group,leonuri low-dose,medium-dose and high-dose groups and dexamethasone positive control group,with 10 mice in each group.Mice in the blank control group was injected with 50 μL PBS buffer vaginally, and the other groups were injected with 50 μL LPS (1 mg/mL) vaginally to establish an animal model of mouse endometritis. After modeling,the low-dose,medium-dose and high-dose groups were intraperitoneally injected with 7.5,15.0 and 30.0 mg/kg leonurine,respectively,while the positive control group was intraperitoneally injected with dexamethasone at a dose of 5.0 mg/kg,once every 6 hours,for 3 consecutive times.The mice were sacrificed 24 hours after modeling,and the uterine index of each group was calculated.The pathological changes of uterine tissue were observed by HE staining.The contents of interleukin-1β (IL-1β),IL-6 and tumor necrosis factor-α (TNF-α) and the activity of myeloperoxidase (MPO) in uterine tissue were determined by ELISA.【Result】 Compared with blank control group,the uterine index,the contents of inflammatory cytokines TNF-α,IL-1β and IL-6 in uterine tissue and the activity of MPO in model group were extremely significantly increased (P＜0.01).Compared with model group,different doses of leonurine could reduce the uterine index,the contents of inflammatory cytokines TNF-α,IL-1β and IL-6 and the activity of MPO in uterine tissue of LPS-induced endometritis (P<0.01) and showed dose-dependent.Compared with model group,uterine index,contents of inflammatory cytokines TNF-α,IL-1β and IL-6 and MPO activity in positive control group were extremely significantly decreased (P＜0.01).【Conclusion 】 Leonurine could reduce the contents of TNF-α,IL-1β,IL-6 and the activity of MPO in mice with LPS-induced endometritis and alleviate inflammatory symptoms,showing a good anti-inflammatory effect.

Key words: leonurine; mice; lipopolysaccharide (LPS); endometritis; inflammatory cytokines

CLC Number:

S853.7

SHAO Yongbin, ZHENG Yichuang, ZHANG Jingyi, WEI Linying, TIAN Yong, SUN Guojie, LIU Yuran, LUO Yan, GU Xinli. Study on the Anti-inflammatory Effect of Leonurine on LPS-induced Endometritis in Mice[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2149-2155.

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Study on the Anti-inflammatory Effect of Leonurine on LPS-induced Endometritis in Mice

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