China Animal Husbandry and Veterinary Medicine ›› 2023, Vol. 50 ›› Issue (2): 693-703.doi: 10.16431/j.cnki.1671-7236.2023.02.027

• Basic Veterinary Medicine • Previous Articles     Next Articles

Study on Immunomodulatory Effect of Prepared Radix Rehmanniae Polysaccharide on Cyclophosphamide-induced Immunosuppression in Mice

XU Qianqian1, YANG Wanqun2, WANG Yubo3, WU Fei2, DAI Juanjuan4, QIN Mimi5, ZHUANG Jinqiu1, WANG Yan1, SHEN Zhiqiang1, LI Jichang6   

  1. 1. Shandong Binzhou Animal Science and Veterinary Medicine Academy, Binzhou 256600, China;
    2. Animal Medicine College, Qingdao Agricultural University, Qingdao 266109, China;
    3. Department of Thoracic Surgery, Binzhou Medical College Hospital, Binzhou 256600, China;
    4. Clinical Study Center, Binzhou Medical College Hospital, Binzhou 256600, China;
    5. Department of Pathology, Binzhou Medical College Hospital, Binzhou 256600, China;
    6. College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China
  • Received:2022-06-17 Online:2023-02-05 Published:2023-02-06

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Abstract: 【Objective】 This study was aimed to investigate the immunomodulatory effects of prepared Radix Rehmanniae polysaccharide (PRRP) on cyclophosphamide (CTX)-induced immunesuppression in mice,and provide scientific evidence for the clinical use of PRRP on immune decrease diseases and development of dietary supplement.【Method】 Thirty six Kunming mice were randomly grouped into six groups:Blank control group,model group, Astragalus polysaccharide (APS) group,and three PRRP groups,which oral administer 50,100,200 mg/kg PRRP as PRRPL,PRRPM and PRRPH groups,respectively,six mice each group.Except normal control group,mice in other groups were given 80 mg/kg CTX via peritoneal injection to induce immunosuppression situation.After model was prepared,the mice were given drugs,once a day,for continually 15 days.The body weight,phagocytosis of peritoneal macrophage,immune organ index,tissue damage of thymus and spleen,splenic lymphocyte proliferation and cell cycle were measured,productions of cytokines in serum were determined,and Peyer’s patches (PPs) in intestinal were also counted.【Result】 Compared with blank control group,the index of spleen in model group was extremely significantly improved (P＜0.01),red and white pulp divided unclear,the number of splenic lymphocyte was decreased;The index of thymus was reduced,structure was destroyed with unclear cortex and medulla;The splenic lymphocyte proliferation,phagocytosis of peritoneal macrophage and interleukin-1 β (IL-1β),IL-6 and γ-interferon (INF-γ) in serum were significantly or extremely significantly decreased (P＜0.05; P＜0.01);The splenic lymphocytes were arrested in G0/G1 stage.Compared with model group,the spleen index in mice was extremely significantly decreased by PRRP (P＜0.01),the thymus and spleen structures were destroyed by CTX,the splenic lymphocyte proliferation was extremely significantly induced by advanced and lipopolysaccharide (LPS) (P＜0.01);The levels of IL-1β,IL-6,tumor necrosis factor α (TNF-α) and INF-γ in serum were significantly or extremely significantly improved (P＜0.05; P＜0.01);The splenic lymphocyte arrest was adjusted,and the phagocytosis of peritoneal macrophage in PRRPH group was significantly improved (P＜0.05).There were no significant changes of PRRP and before and after modeling on the number of PPs in intestinal (P＞0.05).【Conclusion】 PRRP could accelerate recovery of CTX-induced immunosuppression in mice,and could be used as drugs or dietary supplement for the treatment of immune decrease diseases.

Key words: PreparedRadix Rehmanniae Polysaccharide; mice; immunosuppression; immunomodulatory

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