补充牛磺酸对高脂饮食C57BL/6J小鼠糖脂代谢的影响

doi:10.16431/j.cnki.1671-7236.2022.12.010

Abstract

Abstract: 【Objective】 The experiment was conducted to study the effect of taurine on glucose and lipid metabolism in obese mice induced by high-fat diet.【Method】 20 SPF C57BL/6J male mice aged 5 weeks were randomly divided into 4 groups (blank, model, 3% taurine and 5% taurine groups) with 5 mice in each group.The experiment lasted for 15 weeks.The mice in blank group were fed a control diet, the mice in model group were fed a high-fat diet, and the mice in other two groups were supplemented with 3% and 5% taurine respectively on the basis of the model group diet.Oral glucose tolerance test (OGTT) and insulin tolerance test (ITT) were performed 2 weeks before the end of the trial.At the end of the experiment, the eyeballs were anesthetized with ether and blood was collected.The mice were sacrificed by cervical dislocation and the liver, kidney, spleen and epididymal adipose tissue were collected and weighed to calculate the organ/fat coefficient.The serum biochemical indexes and the contents of leptin (LEP) and adiponectin (ADPN), triglyceride (TG) and total cholesterol (TC) in liver were determined.Oil red O staining and hematoxylin-eosin (HE) staining were used to observe the distribution of lipid droplets and the morphological changes of adipocytes in liver.【Result】 ①Compared with blank group, the body weight of mice in model group was extremely significantly increased (P<0.01), and the indexes of blood glucose (GLU) and blood lipids were abnormal.The glucose tolerance and insulin tolerance increased extremely significantly under the curve (P<0.01), which was consistent with the characteristics of the obesity model. ②Compared with model group, the total weight gain, liver coefficient and fat coefficient of taurine supplementation were extremely significantly decreased (P<0.01), the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and content of low density lipoprotein (LDL) in serum were extremely significantly increased (P<0.01), while the content of GLU was significantly decreased (P<0.05). The contents of TG and TC in liver were extremely significantly decreased (P<0.01), the content of ADPN was extremely significantly increased (P<0.01), glucose tolerance and insulin tolerance were positively and extremely significantly decreased under the curve (P<0.01). The number of lipid droplets in liver decreased, and the cross section of adipocytes decreased extremely significantly (P<0.01), the number of adipocytes increased extremely significantly (P<0.01), and the distribution of adipocytes was uniform.【Conclusion】 Taurine might have a protective effect on liver by regulating glucose and lipid metabolism and improving histopathological morphology of liver and fat in obese mice induced by high-fat diet.

Key words: taurine; obese mice; glycolipid metabolism; liver

CLC Number:

Q493

ZHANG Yuanyuan, TIAN Zhichen, SUN Yujia, ZHANG Huimin, YANG Zhangping, WANG Cong, CAO Ke, MAO Yongjiang, LI Mingxun. Effects of Taurine Supplementation on Glycolipid Metabolism in C57BL/6J Mice with High-fat Diet[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4604-4613.

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Effects of Taurine Supplementation on Glycolipid Metabolism in C57BL/6J Mice with High-fat Diet

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