绞股蓝多糖对D-半乳糖致衰老小鼠肝脏抗氧化能力的影响

doi:10.16431/j.cnki.1671-7236.2022.02.007

Abstract

Abstract: 【Objective】 The purpose of this study was to investigate the effect of Gynostemma pentaphyllum polysaccharides (GPP) on the antioxidant capacity of liver in aging mice induced by D-galactose (D-gal).【Method】 Sixty 4-week-old SPF Kunming mice were randomly divided into 6 groups (half male and half female): Blank control group (Control), model group (D-gal), positive control group (Vc), low (50 mg/kg), medium (100 mg/kg) and high (200 mg/kg) dose groups of GPP (GPP-L, GPP-M and GPP-H), with 10 mice in each group.Biochemical, enzyme-linked immunosorbent assay and histological techniques were used to determine the weight change, liver index, T-AOC levels in serum, SOD, GSH-Px and CAT activities, ROS and MDA contents in liver, as well as the liver tissue morphology and liver cord area ratio.【Result】 The weight of mice in each group was an increasing trend, and the weight gain was a decreasing trend, the weight of mice in GPP-M group was lower than that of the other groups, and the weight gain was the least.The liver index and serum T-AOC activity in each dose group of GPP were higher than that in D-gal group.The SOD activity in each dose group of GPP was no significant difference with D-gal group (P > 0.05).The activities of GSH-Px and CAT were increased compared with D-gal group, the GSH-Px activity in GPP-M group and the CAT activity in GPP-L group were significantly higher than that in D-gal group (P < 0.05), and the contents of ROS and MDA in GPP-L group were significantly decreased than that in D-gal group (P < 0.05).HE staining revealed that the cytoplasmic eosinophilia of D-gal group was enhanced, the connection between hepatocytes was loose, and the hepatic sinusoids were dilated significantly, while the liver tissue structure in GPP group was improved, especially GPP-M and GPP-H groups.The proportion of hepatic cord area in GPP-H group was equivalent to that in blank and positive control groups, and the proportion of hepatic cord area in GPP-M and GPP-H groups were significantly higher than that in GPP-L group (P < 0.05).【Conclusion】 GPP could effectively improve the antioxidant capacity of aging mice induced by D-gal and delay the aging, and 100 mg/kg GPP had a significant antioxidant effect on liver of aging mice induced by D-gal.

Key words: Gynostemma pentaphyllum polysaccharide (GPP); liver; D-galactose (D-gal) induced aging; antioxidant

CLC Number:

S852.1

ZHANG Runxiang, BAI Yuting, ZHANG Yinglei, LIU Keke, WANG Xinyu, DONG Yinyu, WANG Xiaoli. Effect of Gynostemma pentaphyllum Polysaccharide on Antioxidative Ability of Liver in Aging Mice Induced by D-galactose[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 462-470.

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Effect of Gynostemma pentaphyllum Polysaccharide on Antioxidative Ability of Liver in Aging Mice Induced by D-galactose

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