发酵杜仲叶对肉鸡生产性能、抗氧化性能和免疫相关基因表达的影响

doi:10.16431/j.cnki.1671-7236.2023.05.012

Abstract

Abstract: 【Objective】 This experiment was aimed to study the effects of Eucommia ulmoides leaves fermented by different probiotics on the performance,antioxidant performance,organ and intestinal immune related gene expression of broilers,so as to provide scientific basis for the research and development of new additives to replace antibiotics.【Method】 In this study,broilers were fed with basal diet (group A), and diets supplied with Eucommia ulmoides leaves fermented by Enterococcus faecium (group B), Eucommia ulmoides leaves fermented by Lactobacillus plantarum (group C) and Eucommia ulmoides leaves mixed fermented by Enterococcus faecium and Lactobacillus plantarum (group D).The average daily feed intake (ADFI),average daily gain (ADG) and feed/gain (F/G) were weighed and calculated on the 28th and 56th days,respectively.The indexes of glutathione peroxidase (GSH-Px),malondialdehyde (MDA) and total antioxidant capacity (T-AOC) in serum were detected by cardiac blood sampling.The spleen,liver and ileal tissues of broilers were collected to detect the expression of gamma interferon (IFN-γ),nitrick oxide synthase (iNOS),interleukin-6 (IL-6) and IL-10 by Real-time quantitative PCR.【Result】 ① Compared with group A,ADG were significantly increased and F/G were significantly decreased in groups B,C and D from 1 to 28 and 1 to 56 d (P＜0.05);ADG in groups B and C were significantly increased,and F/G in groups B,C and D were significantly reduced from 29 to 56 d (P＜0.05).② The activity of GSX-Px in groups B and C were significantly increased at 28 and 56 d (P＜0.05).③ Compared with group A,the mRNA expression of IFN-γ gene in spleen of groups B, C and D and ileum of group D were significantly increased after feeding for 28 d (P＜0.05);The mRNA expression of IFN-γ gene in liver of groups B and D,spleen and cecum of groups B,C and D were significantly increased after feeding for 56 d (P＜0.05).④ Compared with group A,the mRNA expression of IL-6 gene in ileum of groups B and D were significantly decreased after feeding for 28 d (P＜0.05);The mRNA expression of IL-6 gene in liver of group D,spleen of groups C and D,and ileum of groups B and D were significantly decreased after feeding for 56 d (P＜0.05).⑤ Compared with group A,the mRNA expression of IL-10 gene in ileum of groups B and D were increased after feeding for 28 d (P＜0.05);The mRNA expression of IL-10 gene in liver and ileum of groups B,C and D were significantly increased after feeding for 56 d(P＜0.05).⑥ Compared with group A,the mRNA expression of iNOS gene in spleen of group D and ileum of groups B,C and D were significantly increased after feeding for 28 d (P＜0.05);The mRNA expression of iNOS gene in liver and spleen of groups C and D,and ileum of groups B,C and D were significantly increased after feeding for 56 d (P＜0.05).【Conclusion】 Adding different probiotics to ferment Eucommia ulmoides leaves in diet could promote the performance of broilers,and affect the serum antioxidant indexes and intestinal immune related genes.

Key words: broilers; fermented Eucommia ulmoides leaves; production performance; antioxidant properties; immune related genes

CLC Number:

S816.73

QIAO Hongxing, ZHANG Han, ZHAO Shengzhen, SUN Yanting, ZHANG Xiaojing, KANG Yu, BIAN Chuanzhou. Effects of Fermented Eucommia ulmoides Leaves on Production Performance, Antioxidant Properties and Immune Related Gene Expression in Broilers[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1845-1854.

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Effects of Fermented Eucommia ulmoides Leaves on Production Performance, Antioxidant Properties and Immune Related Gene Expression in Broilers

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