产气荚膜梭菌噬菌体及乳酸杆菌联用改善肉鸡生长性能和调节肠道菌群功能研究

doi:10.16431/j.cnki.1671-7236.2023.06.016

Abstract

Abstract: 【Objective】 This study was aimed to determine the combined effects of Clostridium perfringens (C.perfringens) phage and Lactobacillus on the production performance, intestinal structure and microbial diversity of broilers, and provide the prevention and control technique for C.perfringens infection.【Method】 240 1-day-old Blue-footed partridge chickens were divided into 4 groups:HN02 phage group, Lactobacillus group, HN02 phage+Lactobacillus combination group and blank control group, with 6 replicates (10 per replicate) fed 14 days by drinking phage and/or Lactobacillus in water continuously every day during the period.The average daily food intake (ADFI), average daily gain (ADG) and feed-to-weight ratio (F/G) were counted, the organ index were measured.The number of C.perfringens of cecal content was determined and analyzed by 16S rRNA gene amplification for the structure and diversity of intestinal microbial community.【Result】 Compared with blank control group, in HN02 phage+Lactobacillus combination group, the ADG and villus height/crypt depth were significantly increased (P<0.05), the F/G and crypts depth of jejunal and ileum were significantly decreased (P<0.05), and the number of C.perfringens decreased 1.17 lg CFU/mL on 14 days with the best bacteriostatic effect.The results of microbial diversity showed that the relative abundance of Proteobacteria, Firmicutes, Actinobacteriota and Bacteroidota in the cecal content flora of broilers was higher after drinking HN02 phage+Lactobacillus, and the relative abundance of Lactobacillus was higher at the genus level.Compared with blank control group, the relative abundance of Staphylococcus was lower after drinking HN02 bacteriophage+Lactobacillus for 14 days.【Conclusion】 The combination of C.perfringens phage and Lactobacillus could improve the growth performance and intestinal development of broilers, and maintain the diversity of intestinal microbial community, which showed great potential in the prevention of C.perfringens infection in poultry industry.

Key words: Clostridium perfringens; phage; Lactobacillus; broilers; growth performance; intestinal flora

CLC Number:

S852.61

WU Liting, LU Rui, LIU Banhong, BAO Hongduo, WANG Yongjuan, ZHOU Yan, WANG Ran, ZHANG Hui. Combination of Clostridium perfringens Phage and Lactobacillus Improves Growth Performance and Regulates Intestinal Flora Function of Broilers[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2321-2332.

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Combination of Clostridium perfringens Phage and Lactobacillus Improves Growth Performance and Regulates Intestinal Flora Function of Broilers

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