锌、乳酸杆菌对感染沙门氏菌乳鸽免疫及抗氧化功能的影响

doi:10.16431/j.cnki.1671-7236.2023.06.011

Abstract

Abstract: 【Objective】 This experiment was designed to study the effect of zinc and Lactobacillus on the prevention and control of Salmonella infection in squabs.【Method】 Ninety White feather King pigeons of 10 days of age were selected and randomly divided into 5 groups, with 18 pigeons in each group.They were control group (C group), infection group (S group, Salmonella Typhimurium), zinc group (Zn group, zinc sulfate+Salmonella Typhimurium), Lactobacillus group (LAB group, Lactobacillus), and combined treatment group (Zn+LAB group, zinc sulfate+Lactobacillus+Salmonella Typhimurium).On the 5th day of the test, the model of Salmonella Typhimurium challenge was established.The pigeons in group C was fed 1 mL PBS as the control, and other groups were fed 1 mL Salmonella Typhimurium suspension (1×10⁹ CFU/mL) for 3 consecutive days.Blood samples and livers were collected for the determination of immune and antioxidant indexes.Spleen and bursae of Fabricius were collected and weighed to calculate spleen index and bursae index of Fabricius.Jejunum and ileum tissues were collected for the preparation of tissue sections.【Result】 Compared with the control group, the ratio of ileum villus height to crypt depth (V/C) in infected group was significantly decreased (P<0.05).Compared with infection group, the monocyte ratio of squab were significantly decreased in zinc group and Lactobacillus group (P<0.05), and jejunum V/C, ileum V/C and serum T-SOD activities were significantly increased in combined treatment group (P<0.05).【Conclusion】 The systemic infection of Salmonella in squabs could be inhibited by increasing spleen organ index and antioxidant capacity, and improving intestinal morphology by instillation of zinc or combined administration of low dose zinc and Lactobacillus, and the combined administration effects were better.

Key words: zinc; Lactobacillus; Salmonella Typhimurium; squabs; immune; antioxidant

CLC Number:

S816.7

WANG Yangyang, QIN Shizhen, TANG Defu, SHAO Yuxin, SHI Zhaoguo, WANG Zheng, LI Xing, ZHAO Dongdong. Effects of Zinc, Lactobacillus on Immune and Antioxidant Function of Squabs Infected with Salmonella[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2276-2285.

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Effects of Zinc, Lactobacillus on Immune and Antioxidant Function of Squabs Infected with Salmonella

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