丙酮酸钠对山羊冷冻精子质量的影响

doi:10.16431/j.cnki.1671-7236.2023.06.020

Abstract

Abstract: 【Objective】 The aim of this study was to explore the effects of sodium pyruvate on the quality of frozen sperm of goat.【Method】 Semen of 6 Yunshang Black goats was collected by pseudoginosis method, and diluted with Optidyl diluent containing 0, 1, 5, 10 and 20 μmol/L sodium pyruvate.The total motility (TM) and progressive motility (PM) were measured by SCA automatic semen analyzer, the plasma membrane integrity was measured by hypotonic tolerance test (HOST).Acrosomal integrity, phosphatidylserine (PS) distribution and reactive oxygen species (ROS) were measured by flow cytometry.【Result】 The results showed that compared with 0 μmol/L sodium pyruvate group, the TM and PM indices of sperm in 1 and 5 μmol/L sodium pyruvate groups were extremely significantly improved (P<0.01), but there was no significant difference among other treatment groups (P>0.05). Curvilineal velocity and average velocity, slide swing, whip times, plasma membrane integrity, acrosome integrity rate, ROS level of sperm in 1 and 5 μmol/L sodium pyruvate groups were extremely significantly or significantly increased (P<0.01 or P<0.05). Flow analysis results showed that the proportion of normal sperm was significantly higher than that in 0 μmol/L sodium pyruvate group when the concentration of sodium pyruvate was 1, 5 and 10 μmol/L (P<0.05).【Conclusion】 In this study, adding sodium pyruvate into diluent could effectively reduce the damage to goat sperms during freezing and thawing, and significantly improve the locomotor ability, structural integrity and antioxidant activity of frozen sperms.A small amount of sodium pyruvate showed an improvement effect on sperm, while an excessive amount of sodium pyruvate showed a negative effect.The optimum concentration was 1 μmol/L in this experiment.

Key words: goat semen; sodium pyruvate; freeze; antioxidant; reactive oxygen species

CLC Number:

S814.8

LYU Chunrong, LIANG Jiachong, SHAO Qingyong, WU Guoquan, ZHANG Yumei, QUAN Guobo. Effects of Sodium Pyruvate on the Quality of Frozen Goat Sperm[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2363-2369.

References

[1] LV C R, WU G Q, HONG Q H, et al.Spermatozoa cryopreservation:State of art and future in small ruminants[J].Biopreservation and Biobanking, 2019, 17(2):171-182.
[2] RODRÍGUEZ-MARTÍNEZ H, PEÑA VEGA F.Semen technologies in domestic animal species[J].Animal Frontiers, 2013, 3(4):26-33.
[3] LV C R, ALLAI L, SAMEEULLAH M, et al.The effects of antifreeze protein Ⅲ supplementation on the cryosurvival of goat spermatozoa during cryopreservation[J].Biopreservation and Biobanking, 2021, 19(4):298-305.
[4] PURDY P H.A review on goat sperm cryopreservation[J].Small Ruminant Research, 2006, 63(3):215-225.
[5] 李敏, 马凌云, 陈超阳, 等.丙酮酸钠药理学作用的研究现状[J].中国临床药理学杂志, 2021, 37(3):341-344. LI M, MA L Y, CHEN C Y, et al.Research status on the pharmacological effects of sodium pyruvate[J].The Chinese Journal of Clinical Pharmacology, 2021, 37(3):341-344.(in Chinese)
[6] VOTTO J, BOWEN J, BARTON R, et al.Inhaled sodium pyruvate improved FEV1 and decreased expired breath levels of nitric oxide in patients with chronic obstructive pulmonary disease[J].Journal of Aerosol Medicine and Pulmonary Drug Delivery, 2008, 21(4):329-334.
[7] MALLET R T, SUN J, KNOTT E M, et al.Metabolic cardioprotection by pyruvate:Recent progress[J].Experimental Biology and Medicine, 2005, 230(7):435-443.
[8] KAMI K, FUJITA Y, IGARASHI S, et al.Metabolomic profiling rationalized pyruvate efficacy in cybrid cells harboring MELAS mitochondrial DNA mutations[J].Mitochondrion, 2012, 12(6):644-653.
[9] TAKUMI K, AZUMI S, KANA K, et al.Successful treatment of infantile-onset ACAD9 related cardiomyopathy with a combination of sodium pyruvate, beta-blocker, and coenzyme Q10[J].Journal Pediatric Endocrinol & Metabolism, 2019, 32(10):1181-1185.
[10] KORKMAZ F, MALAMA E, SIUDA M, et al.Effects of sodium pyruvate on viability, synthesis of reactive oxygen species, lipid peroxidation and DNA integrity of cryopreserved bovine sperm[J].Animal Reproduction Science, 2017, 185:18-27.
[11] BILODEAU J F, BLANCHETTE S, CORMIER N, et al.Reactive oxygen species-mediated loss of bovine sperm motility in egg yolk Tris extender:Protection by pyruvate, metal chelators and bovine liver or oviductal fluid catalase[J].Theriogenology, 2002, 57:1105-1122.
[12] 吴皛皛, 李楠, 段洪云, 等.抗坏血酸和丙酮酸钠对马精液低温保存效果的影响[A].中国畜牧兽医学会马学分会成立大会学术论文集[C].2014. WU X X, LI N, DUAN H Y, et al.Effect of ascorbic acid and sodium pyruvate on cryopreservation of horse semen[A]. Collected Papers of the Founding Conference of the Chinese Society of Animal Husbandry and Veterinary Medicine[C].2014.(in Chinese)
[13] 金美林, 蔡元.丙酮酸钠对鸡精液稀释保存效果的影响[J/OL].畜牧兽医科学(电子版), 2017, 7:2. JIN M L, CAI Y.Effect of sodium pyruvate on dilution preservation of chicken semen[J/OL].Animal Husbandry and Veterinary Science (Electronic Version), 2017, 7:2.(in Chinese)
[14] BREININGER E, BECONI M T.Ascorbic acid or pyruvate counteracts peroxidative damage in boar sperm cryopreserved with or without α-tocopherol[J].Animal Science Papers and Reports, 2014, 32:15-23.
[15] 胡传活, 杨俊涛, 梁琪妹, 等.丙酮酸盐对牛精液冷冻效果的影响[J].河南农业科学, 2006, 7:105-108. HU C H, YANG J T, LIANG Q M, et al.Effect of pyruvate on cryopreservation of bull semen[J].Journal of Henan Agricultural Sciences, 2006, 7:105-108.(in Chinese)
[16] CHEN F, GAO L, ZHOU H, et al.The association between sperm head elongation and semen quality[J].Andrology, 2019, 7(6):840-845.
[17] SILVESTRE M A, YÁNIZJ L, PEA F J, et al.Role of antioxidants in cooled liquid storage of mammal spermatozoa[J].Antioxidants (Basel), 2021, 10(7):1096.
[18] GONZÁLEZ-MARÍN C, GOSÁLVEZ J, ROY R, et al.Types, causes, detection and repair of DNA fragmentation in animal and human sperm cells[J].International Journal of Molecular Sciences, 2012, 13(11):14026-14052.
[19] TATONE C, EMIDIO G D, VENTO M, et al.Cryopreservation and oxidative stress in reproductive cells[J].Gynecological Endocrinology, 2010, 26(8):563-567.
[20] HERENG T H, ELGSTOEN K B P, CEDERKVIST F H, et al.Exogenous pyruvate accelerates glycolysis and promotes capacitation in human spermatozoa[J]. Human Reproduction, 2011, 26(12):3249-3263.
[21] SEIFI-JAMADI A, KOHRAM H, ZARE-SHAHNEH A, et al.Quercetin ameliorate motility in frozen-thawed turkmen stallions sperm[J].Journal of Equine Veterinary Science, 2016, 45:73-77.
[22] CARRASQUEL MARTÍNEZ G, ALDANA A, CARNEIRO J, et al.Acrosomal alkalinization occurs during human sperm capacitation[J]. Molecular Human Reproduction, 2022, 3:1-18.
[23] JUMINTONO J, ALKUBAISY S, SILVA D Y, et al.Effect of cystamine on sperm and antioxidant parameters of ram semen stored at 4 ℃ for 50 hours[J].Archives of Razi Institute, 2021, 76(4):1115-1123.
[24] ALLAI L, BENMOULA A, DA-SILVA M, et al.Supplementation of ram semen extender to improve seminal quality and fertility rate[J].Animal Reproduction Science, 2018, 192:6-17.
[25] DESAGHER S, GLOWINSKI J, PREMONT J.Pyruvate protects neurons against hydrogen peroxide-induced toxicity[J].The Journal of Neuroscience, 1997, 17(23):9060-9067.

Effects of Sodium Pyruvate on the Quality of Frozen Goat Sperm

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Yangyang, QIN Shizhen, TANG Defu, SHAO Yuxin, SHI Zhaoguo, WANG Zheng, LI Xing, ZHAO Dongdong. Effects of Zinc, Lactobacillus on Immune and Antioxidant Function of Squabs Infected with Salmonella [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2276-2285.
[2]	LI Yongqiang, BAO Ruiying, WANG Qin, QIU Pingfei, LI Xiaochun, SHI Huiyu, ZHANG Haiwen, WANG Xuemei. Protective Effects of Antioxidant Peptides from Maggot on Oxidative Stress Injury of IPEC-J2 Cells [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1836-1844.
[3]	QIAO Hongxing, ZHANG Han, ZHAO Shengzhen, SUN Yanting, ZHANG Xiaojing, KANG Yu, BIAN Chuanzhou. Effects of Fermented Eucommia ulmoides Leaves on Production Performance, Antioxidant Properties and Immune Related Gene Expression in Broilers [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1845-1854.
[4]	LEI Qi, REN Jie, KANG Jie. Effects of Scutellaria baicalensis georgi on Antioxidant Capacity,Sex Hormone Levels and mRNA Expression of ERα in Female Mice Exposed to ZEA [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 989-997.
[5]	ZHAO Luyuan, ZOU Chenhao, CAO Jiu'ai, GUO Yanshan, LIU Lijun, ZHAN Xiu'an, LIN Gang, LIU Bing, YU Dongyou. Effects of Low Level of Compound Organic Trace Minerals on Serum Biochemistry,Immune Function and Antioxidant Capacity of Meat Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 86-96.
[6]	ZHONG Hualing, HE Congcong, WU Bangyuan. Effects of Fresh Alfalfa and Full-price Pellet Feed on the Antioxidant Properties and Muscle Structure of Rabbit Stomach [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 143-150.
[7]	WANG Shuaijie, TAN Zihan, LI Hanbo, SUN Xiangxue, AI Sihan, WANG Lei, ZHAO Di, HOU Yongqing. Effects of Quercetin on Intestinal Morphology, Antioxidant Function and Jejunal Lipid Metabolism Gene Expression in Piglets with Porcine Epidemic Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3391-3399.
[8]	WEI Jia, BAI Qin, LUO Xiaolin, GUAN Jiuqiang, AN Tianwu, ZHAO Hongwen, TAN Wu, LI Huade, XIE Rongqing, SHA Quan, JIANG Mingfeng, ZHANG Xiangfei. Effects of Different Weaning Strategies on Growth, Serum Biochemical Indexes and Antioxidant Capacity of Yak Calves [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3400-3410.
[9]	LI Yipeng, ZHANG Hu, REN Juan, REN Xin, LIU Juxiang, WANG Gengnan. Study on the Extraction Process of Chicoric Acid from Echinacea Root and Its Protective Effects on Oxidative Stress Damaged Intestinal Cells [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2497-2505.
[10]	LI Hongying, HE Qin, HUANG Enfu, LIANG Qi. Effects of Plant Essential Oils on Immune Function, Antioxidant Capacity and Gut Microbiota of Shan Partridge Ducks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2586-2592.
[11]	LIU Jiajia, HE Linjiao, JING Hongxin, HAN Tao, MA Lixin, LI Xiaobin. Effects of Supplemental Formononetin on Performance,Blood Gas and Antioxidant Indexes of Yili Horse in 1 000 m Race [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1738-1745.
[12]	ZHANG Yuanxin, WANG Yanyu, YUN Yanhong, CHEN Junpu, SHI Huiyu, WANG Xuemei. Establishment of Oxidative Damage Model of Goat Mammary Epithelial Cells [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1917-1925.
[13]	ZHANG Rui, ZHOU Weiqiang, MA Yue, WU Jianping, LEI Zhaomin. Effects of Oregano Essential Oil and Colostrum Complex on Growth Performance, Serum Indicators Related Antioxidant and Immune in Opportunity Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1314-1321.
[14]	WANG Chaopu, MA Xianyong, TIAN Zhimei, YU Miao, LIU Zhichang, DENG Dun, RONG Ting, TI Junling, SONG Min, ZHANG Huihua. Effects of Compound Chinese Medicine Fermentation Powder on Growth Performance,Nutrient Apparent Digestibility,Antioxidant Function and Immune Function of Weaned Piglets [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1322-1331.
[15]	WU Yuan, QIN Xin, DONG Yuanyang, HAN Miaomiao, MIAO Zhiqiang, WEI Qingyu, SONG Xianyi, SHEN Liyan, FENG Yan, LI Huifeng, LI Jianghui. Effects of Dietary Methionine on Growth Performance, Antioxidant Capacity and Stress Tolerance of Broilers Under High-density Feeding [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 913-923.