紫锥菊根中菊苣酸提取工艺及对氧化应激肠道细胞的保护作用研究

doi:10.16431/j.cnki.1671-7236.2022.07.007

Abstract

Abstract: 【Objective】 The extraction process of cichoric acid from Echinacea root was optimized,and the antioxidant effect of cichoric acid in intestinal cells was determined.【Method】 In the experiment,the economical organic solvent ethanol was selected as the extraction solvent of Echinacea root powder (80 mesh),and several factors such as extraction solvent concentration,extraction temperature,extraction time,extraction times,and the ratio of raw materials to solvent were set for analysis,and the results of each factor were obtained.The corresponding curve of acid yield and this factor,and then on the basis of single factor,select solvent concentration (30%,40%,50%),extraction temperature (60,70,80 ℃),extraction time (1,2,3 h),solid-liquid ratio (1∶12,1∶15,1∶18),four factors and three levels of orthogonal experiments were designed.With different concentrations of H₂O₂ (100,200,400,600,800,1 000 μmol/L) to treat human colon adenocarcinoma cells (Caco-2) and porcine small intestinal epithelial cells (IPEC-J2).The cells before and after oxidative stress were treated with chicory acid (0,100,200,400,600,800,1 000 μmol/L) for 24 h respectively.The protective and alleviating effects of chicory acid on oxidative stress of two intestinal cells were measured by MTT method.【Result】 The results of extraction process showed the optimal extraction conditions of chicoric acid were 50% ethanol at 70 ℃ with a solid-liquid ratio of 1∶18,ultrasonic-assisted extraction for 2 h.Finally,the yield of this process was 1.89 mg/g.The results of cellular antioxidant assay showed that chicoric acid had preventive and alleviating effects on both Caco-2 and IPEC-J2 cell lines which damaged by oxidative stress.The IC₅₀ values of its protective and alleviating effects were 172.6 and 207.5 μmol/L for Caco-2,133.1 and 196.5 μmol/L for IPEC-J2,respectively.【Conclusion】 The best extraction process of chicoric acid from Echinacea root powder was obtained,which provided a reference for industrial and efficient extraction of chicoric acid,and the cell antioxidant experiment showed the intestinal antioxidant ability of chicoric acid.The calculated IC₅₀value of its protective effect was smaller than that of the mitigation effect.It was further proved that chicoric acid had the potential as a future intestinal antioxidant drug.

Key words: Echinacea; chicoric acid; extraction; intestinal cells; antioxidant effect

CLC Number:

S859.7

LI Yipeng, ZHANG Hu, REN Juan, REN Xin, LIU Juxiang, WANG Gengnan. Study on the Extraction Process of Chicoric Acid from Echinacea Root and Its Protective Effects on Oxidative Stress Damaged Intestinal Cells[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2497-2505.

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Study on the Extraction Process of Chicoric Acid from Echinacea Root and Its Protective Effects on Oxidative Stress Damaged Intestinal Cells

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