黄芩多糖的提取及结构表征

doi:10.16431/j.cnki.1671-7236.2023.06.036

Abstract

Abstract: 【Objective】 The purpose of the test was to explore the optimal extraction conditions of Scutellaria baicalensis polysaccharides by water extraction and alcohol precipitation, improve the extraction rate of Scutellaria baicalensis polysaccharide, and explore the structure of Scutellaria baicalensis polysaccharide, so as to provide the basis for industrial extraction of Scutellaria baicalensis polysaccharide.【Method】 The effects of extraction time, extraction temperature, material-to-liquid ratio and extraction times on the extraction yield of polysaccharide were investigated by single factor test.Box-Behnken central combination experimental design method was used to select the optimal extraction time, extraction temperature, material-to-liquid ratio and extraction times, the extraction rate of polysaccharide was the response value (the design of four factors and three levels test), through the response surface analysis to determine the optimal extraction conditions of Scutellaria baicalensis polysaccharide extraction by water extraction and alcohol precipitation method.The polysaccharide was separated and purified by column chromatography.The group, purity, molecular weight and monosaccharide composition of Scutellaria baicalensis polysaccharide were analyzed by infrared spectrum, ultraviolet spectrum, high performance liquid chromatography (HPLC) and gel chromatography.【Result】 Response surface analysis determined that the extraction temperature was 98 ℃, the material-to-liquid ratio was 1:12.40 (g/mL), the extraction time was 138 min, and the extraction times were 4, the extraction yield of crude polysaccharide was 12.23%.Hq-3-1 was purified by DEAE-52 cellulose column chromatography and dextran G-100 gel column chromatography.Gel permeation chromatography analysis showed that Hq-3-1 was a homogeneous polysaccharide with a molecular weight of 58 794 u.The monosaccharides of Hq-3-1 components were analyzed by pre-column derivatization by HPLC.The results showed that the monosaccharides contained fructose, mannose, rhamnose-glucuronic acid, galactose, arabinose and fucose.The UV absorption map showed that Hq-3-1 had no absorption peak at 260 and 280 nm, indicating that it did not contain nucleic acid and protein.Infrared spectrum analysis showed that Hq-3-1 had a polysaccharide characteristic absorption peak, which was α-D-mannose-pyranoid heteropoly.【Conclusion】 Under the optimal extraction conditions, the extraction rate of Scutellaria baicalensis polysaccharides by water extraction and alcohol precipitation was 12.23%.This method was simple, convenient, and had a high extraction rate.This study proved that Scutellaria baicalensis polysaccharide was a heteropolysaccharide with a molecular weight of 58 794 u.

Key words: Scutellaria baicalensis polysaccharide; extraction process; separation and purification; structural analysis

CLC Number:

S859.3

LIU Peng, LI Jingjing, QIAO Yanliang, ZHU Lianqin, ZHU Fenghua. Extraction and Structure Characterization of Scutellaria baicalensis Polysaccharide[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2518-2530.

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Extraction and Structure Characterization of Scutellaria baicalensis Polysaccharide

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