牡荆总黄酮提取工艺优化及其对产气荚膜梭菌抑菌活性研究

doi:10.16431/j.cnki.1671-7236.2022.06.040

Abstract

Abstract: 【Objective】 This study was aimed to explore the extraction technology of total flavonoids from Vitex negundo L. var. cannabifolia (Sieb. et Zucc.) Hand.-Mazz. (V. negundo) and its antibacterial activity against Clostridium perfringens.【Method】 Rutin was used as reference substance and the content of total flavonoids in V. negundo was determined by spectrophotometry.Taking the content of total flavonoids as the investigation index, the effects of ethanol concentration, extraction time, extraction temperature and solid-liquid ratio on the extraction amount of total flavonoids from V. negundo were studied through single factor test, and the level range of the best process under the condition of single factor was determined.On the basis of single factor test, through the design of L9 (3⁴) orthogonal test, the content of total flavonoids in V. negundo was determined by NaNO₂-AL(NO₃)₃-NaOH color development method, the standard curve was drawn, the regression equation was calculated, and the standard curve was verified through the methodological investigation of precision, stability, repeatability and sample recovery.By substituting the data obtained by the experiment into the standard curve, the best preparation process of total flavonoids of V. negundo was obtained, and on this basis, the antibacterial activity of its extract against Clostridium perfringens was investigated by micro double dilution method.【Result】 The optimum extraction conditions of total flavonoids from V. negundo were ethanol concentration of 60%, extraction temperature of 50 ℃, extraction time of 2.5 h and solid-liquid ratio of 1:35.Under the optimum extraction conditions, the content of total flavonoids in V. negundo was the highest (43.17 mg/g).The order of influencing factors was solid-liquid ratio > extraction time > ethanol concentration > extraction temperature.The solid-liquid ratio had the greatest influence on the content of total flavonoids in V. negundo.The minimum inhibitory concentration (MIC) of V. negundo extract against Clostridium perfringens was 7.81 μg/mL.【Conclusion】 Under the conditions of ethanol concentration of 60%, extraction temperature of 50 ℃, extraction time of 2.5 h and solid-liquid ratio of 1:35, the content of total flavonoids extracted from V. negundo was the highest.The prepared extract had strong antibacterial effect on Clostridium perfringens, which provided a theoretical basis for the production of high-quality V. negundo extract.

Key words: Vitex negundo L. var. cannabifolia (Sieb. et Zucc.) Hand.-Mazz. (V. negundo); total flavonoids; extraction process; MIC

CLC Number:

S853.7

LI Yahui, LUO Shengjun, TANG Xinggang, YUAN Minggui, WEI Qilin, LOU Hua, XIANG Rong. Study on Optimization of Extraction Process and Antibacterial Activity to Clostridium Perfringens of Total Flavonoids from Vitex negundo L.var. cannabifolia (Sieb.et Zucc.) Hand.-Mazz.[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2406-2413.

References

[1] 夏诗琪, 王培玲, 张丽, 等.中草药饲料添加剂的抑菌研究及应用现状[J].江西农业大学学报, 2020, 42(6):1231-1236. XIA S Q, WANG P L, ZHANG L, et al.A Study of bacteriostasis of Chinese herbal medicine feed additive and its application status[J].Acta Agriculturae Universitatis Jiangxiensis, 2020, 42(6):1231-1236.(in Chinese)
[2] 凌玮玮.牡荆化学成分及其抑菌活性研究[D].合肥:安徽农业大学, 2010. LING W W.Constituents and antibacterial activities of Vitex negundo Linn. var. Cannabifolia[D].Hefei:Anhui Agricultural University, 2010.(in Chinese)
[3] 易丹, 侯永清.天然植物活性成分与猪肠道健康的研究进展[J].动物营养学报, 2020, 32(10):4518-4524. YI D, HOU Y Q.Research progress on natural plant bioactive compounds and swine intestinal health[J].Chinese Journal of Animal Nutrition, 2020, 32(10):4518-4524.(in Chinese)
[4] 刘华, 刘秀斌, 吴俊, 等.饲用植物及其提取物在饲料替抗中的应用[J].饲料工业, 2020, 41(12):20-24. LIU H, LIU X B, WU J, et al.The application of forage natural plants and their extracts in growth-promoting antibiotics replacements[J].Feed Industry, 2020, 41(12):20-24.(in Chinese)
[5] 林艳英, 麦志雄, 黄罕妮, 等.牡荆属植物化学成分及质量控制方法的研究进展[J].当代医药论丛, 2014, 12(4):141-143. LIN Y Y, MAI Z X, HUANG H N, et al.The research progress of plant chemical composition and quality control methods of Peony Jing genus[J].Contemporary Medical Symposiu, 2014, 12(4):141-143.(in Chinese)
[6] 王秀杰.广西牡荆抗炎活性成分研究及Telekin的结构修饰[D].济南:山东大学, 2015. WANG X J.Anti-inflammatory constituents of Vitex kwangsiensia and synthesis of B-amino telekin derivatives[D].Jinan:Shandong University, 2015.(in Chinese)
[7] 周燕, 何蓉蓉, 邱峰, 等.牡荆属植物的研究进展[J].中国实验方剂学杂志, 2010, 16(17):229-233. ZHOU Y, HE R R, QIU F, et al, Research advances on Vitex L.[J].Chinese Journal of Experimental Traditional Medical Formulae, 2010, 16(17):229-233.(in Chinese)
[8] 李华强.牡荆子的化学成分研究以及生药学鉴定[D].上海:上海师范大学, 2015. LI H Q.Chemical constituents and pharmacognostical identification of Vitex negundo var.Cannabifolia fruits[D].Shanghai:Shanghai Nomal University, 2015.(in Chinese)
[9] 刘浩, 唐红枫.鱼腥草中总黄酮的提取工艺优化研究[J].湖北农业科学, 2019, 58(21):155-158. LIU H, TANG H F.Optimize procedure to extract the flavonoids from Houstonian cordata[J].Hubei Agricultural Sciences, 2019, 58(21):155-158.(in Chinese)
[10] 向蓉, 何立美, 陈文露, 等.辣蓼总黄酮提取工艺筛选及优化[J].中国兽药杂志, 2019, 53(2):40-47. XIANG R, HE L M, CHEN W L, et al.Screening and optimizationg of extraction of flavonoids from polygonum hydropiper[J].Chinese Journal of Veterinary Drug, 2019, 53(2):40-47.(in Chinese)
[11] 黄丽芸, 邓鼎森, 陈小兵.黄酮类化合物常用提取方法研究[J].中国药物经济学, 2014, 9(S1):57-58. HUANG L Y, DENG D S, CHEN X B.Research on common extraction methods of Flavonoids[J].China Journal of Pharmaceutical Economics, 2014, 9(S1):57-58.(in Chinese)
[12] 艾芹.油茶主要病害植物源杀菌剂的研制[D].长沙:中南林业科技大学, 2015. AI Q.Study on botanical fungicides against the major diseases of Camellia oleifera[D].Changsha:Central South University of Forestry and Technology, 2015.(in Chinese)
[13] 梁海运, 马泽盟, 孙瑞良, 等.植物源黄酮抑菌作用研究进展[J].日用化学工业, 2021, 51(8):775-781. LIANG H Y, MA Z M, SUN R L, et al.Research progress on antibacterial activity of bioactive flavonoids[J].China Surfactant Detergent & Cosmetics, 2021, 51(8):775-781.(in Chinese)
[14] 向蓉, 舒柄垚, 高原, 等.牡荆提取物主要化学成分分析及自由基清除能力[J].中国兽医学报, 2021, 41(6):1146-1153. XIANG R, SHU B Y, GAO Y, et al.Chemical constituents and free radical scavenging ability of Vitex extract[J].Chinese Journal of Veterinary Science, 2021, 41(6):1146-1153.(in Chinese)
[15] 孙阿宁, 任改艳, 邓超, 等.牡荆素减轻小鼠溃疡性结肠炎的药效作用及机制研究[J].中国药理学通报, 2014, 30(12):1677-1681. SUN A N, REN G Y, DENG C, et al.Effects and mechanisms of Vitexin against ulcerative colitis in mice[J].Chinese Pharmacological Bulletin, 2014, 30(12):1677-1681.(in Chinese)
[16] 吴远彬, 李建凤.黄酮类化合物提取及其对家禽的影响[J].山东化工, 2021, 50(2):146-147. WU Y B, LI J F.Extraction of flavonoids and its effect on poultry[J].Shandong Chemical Industry, 2021, 50(2):146-147.(in Chinese)
[17] 王霄然.核桃内种皮废弃物的再生利用[D].天津:天津科技大学, 2017. WANG X R.The regeneration utilization of walnut (Juglans regia L.) kernelpellicle) by-product[D].Tianjin:Tianjin Univercity of Science and Technology, 2017.(in Chinese)
[18] 代现平, 班翠红, 韩景田.正交设计研究山牡荆总黄酮提取工艺[J].安徽农业科学, 2014, 42(23):7748-7749. DAI X P, BAN C H, HAN J T.Extraction technique for total flavonoids from vitex quinate by orthogonal test[J].Journal of Anhui Agricultural Sciences, 2014, 42(23):7748-7749.(in Chinese)
[19] 黄琼.牡荆挥发油和黄酮提取工艺及其生理活性研究[D].南宁:广西大学, 2006. HUANG Q.Studu on the extraction technology and biological activities of volatileoil and flavone from Vitex negundo L.Var.Cannabifolia (Sieb.Etaucc.) Hand-mazz[D].Nanning:Guangxi Univerciyt, 2006.(in Chinese)
[20] 王楠, 苏誉, 刘文杰, 等.植物内生菌中抗耐药微生物活性成分的研究进展[J].生物技术通报, 2021, 37(8):263-274. WANG N, SU Y, LIU W J, et al.Research progress on active compounds against drug-resistant microorganism from plant endophytes[J].Biotechnology Bulletin, 2021, 37(8):263-274.(in Chinese)
[21] 钟宜科, 吴迪, 花晓丹, 等.黄酮类化合物抑菌作用研究进展[J].中国食品添加剂, 2019, 30(8):166-171. ZHONG Y K, WU D, HUA X D, et al.Progress in the study of antibacterial effect of flavonoids compounds[J].China Food Additives, 2019, 30(8):166-171.(in Chinese)
[22] 袁伟康, 戚南山, 廖申权, 等.影响鸡坏死性肠炎病例复制的关键因素[J].中国兽医学报, 2021, 41(1):186-191. YUAN W K, QI N S, LIAO S Q, et al.Major factors influencing the experimental reproduction of necrotic enteritis in chickens[J].Chinese Journal of Veterinary Science, 2021, 41(1):186-191.(in Chinese)
[23] 郭妍妍, 曹伟胜.AGP禁用形势下如何维护家禽肠道健康[J].养禽与禽病防治, 2020, 8:2-5. GUO Y Y, CAO W S.Maintaining poultry intestinal health under the situation of AGP prohibition[J].Poultry Husbandry and Disease Control, 2020, 8:2-5.(in Chinese)
[24] 段凯文, 黄小红, 邓海英, 等.中药及组方对产气荚膜梭菌的体外抑菌作用[J].江西畜牧兽医杂志, 2019, 4:37-39. DUAN K W, HUANG X H, DENG H Y, et al.In vitro bacteriostatic effect of Chinese herbs and their prescriptions on Clostridium perfringens[J].Jiangxi Journal of Animal Husbandry & Veterinary Medicine, 2019, 4:37-39.(in Chinese)
[25] 苏皓瑀, 刘春海, 韩建林, 等.酸化剂及其衍生物与植物精油复合对常见致病菌体外抑菌效果研究[J].饲料博览, 2021, 5:5-9. SU H Y, LIU C H, HAN J L, et al.Study on in vitro antibacterial effect of acidulant and its derivatives combined with plant Essential oil on common pathogenic bacteria[J].Feed Review, 2021, 5:5-9.(in Chinese)

Study on Optimization of Extraction Process and Antibacterial Activity to Clostridium Perfringens of Total Flavonoids from Vitex negundo L.var. cannabifolia (Sieb.et Zucc.) Hand.-Mazz.

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LI Yanghui, XU Tao, ZHANG Shuxian, LIU Ke, JIA Tianqing, CHEN Ning, YIN Junliang, XI Linqiao, WANG Dong, ZHOU Xiaoling. Effect of Replacing Wheat Straw with Cumin Straw on Growth Performance, Slaughter Performance and Plasma Biochemical Parameters of Lambs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2296-2304.
[2]	XU Ziyan, JIANG Yang, LIU Xuetao, LIU Bohan, ZHANG Wen, YAN Changguo, CUI Lianhua. Preparation of Pectin/Chitosan Curcumin Microcapsules and Its Study on Simulated Gastrointestinal Environments [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2312-2320.
[3]	SHI Qiuying, DUAN Baomin, LI Ying, NIU Dengyun, GENG Chenyang, ZHOU Jiawei, WANG Rui, FENG Jingjing. Analysis of Genetic Variation of S1 Gene of Porcine Epidemic Diarrhea Virus in Some Areas of China from 2020 to 2021 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2468-2478.
[4]	LIU Peng, LI Jingjing, QIAO Yanliang, ZHU Lianqin, ZHU Fenghua. Extraction and Structure Characterization of Scutellaria baicalensis Polysaccharide [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2518-2530.
[5]	ZHANG Xiaomeng, JIAO Anhui, WANG Yuqi, FAN Nanzhu, JIN Qingguo. Effects of Rosiglitazone Supplementation on Redox Homeostasis of Oocytes in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1818-1827.
[6]	LU Hui, CAI Gaofeng, WU Caihong, QIU Shulei, YUAN Chen, CHEN Xiaolan, LIU Yun, QIN Kaili. Effect of Agrocybe aegerita Polysaccharid on Immunomodulatory of Splenic Lymphocytes in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1828-1835.
[7]	OUYANG Kun, WEI Limin, SUN Ruiping, XIE Yali, QI Qi, LI Xiang, LIU Quanwei, CHAO Zhe, ZHANG Yongliang, CHEN Ting. Effects of Curcumin on Growth Performance,Feed Apparent Digestibility and Serum Biochemical Indexes in Hainan Tunchang Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1867-1875.
[8]	LONG Feng, XIE Shouyu, CUI Pengfei, SHI Kaichuang, WEI Xiankai, FENG Shuping, QU Sujie, LU Wenjun, LI Jianfeng, YIN Yanwen, DENG Guohua. Genetic Evolutionary Dynamics Analysis of HA and NA Genes of H9 Subtype Avian Influenza Virus in Guangxi from 2016 to 2021 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1947-1958.
[9]	KANG Pu, REN Zhaowen, CAI Rujian, LYU Zongji, HUANG Yuhao, WANG Xiaohu. Variation Regularity and Transmission of PRRSV-2 Lineage 8 Strains in Guangdong and Surrounding Provinces [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2001-2011.
[10]	CHEN Xi, LI Xinyi, CHEN Fashun, DUAN Yong, DONG Ling, WU Shujie, FAN Jiancheng, XIANG Bin, YANG Liangyu, ZHANG Limei. Study on the Probiotic Properties and Antimicrobial Activity of 5 Strains of Bacillus amyloliquefaciens [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2082-2091.
[11]	XU Chenghui, ZHANG Xumei, YANG Tong, LI Jiahui, SUN Yawei, SHI Huijun, FU Qiang, YANG Li. Molecular Mechanism of Leonurine in the Treatment of Intestinal Inflammation Based on Network Pharmacology and Molecular Docking [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2103-2113.
[12]	SHAO Yongbin, ZHENG Yichuang, ZHANG Jingyi, WEI Linying, TIAN Yong, SUN Guojie, LIU Yuran, LUO Yan, GU Xinli. Study on the Anti-inflammatory Effect of Leonurine on LPS-induced Endometritis in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 2149-2155.
[13]	TANG Yu, YANG Yifeng, ZHANG Ying, XUE Hailong, FENG Huailiang, XU Baozeng. Cytological Study on Oocyte Maturation of Raccoon Dog [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1434-1443.
[14]	WANG Leping, PAN Yan, TAN Yizhou, LI Lei, HAN Kaiou, CAO Yajie, LI Jun, ZHOU Yuqing, LIAO Yuying, MA Dongxin, GONG Yu, PENG Hao, WANG Xiaoye. Isolation,Identification and Genome Analysis of Escherichia coli Phage vB_EcoP_GN07 [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1522-1531.
[15]	HUO Mingkai, GUAN Feihu, QIU Runhui, WEI Chunyan, YU Haitao, ZHU Jiale, ZHANG Hui. Study on the Mechanism of DDX1 Inhibiting Porcine Epidemic Diarrhea Virus Replication [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1556-1566.