果胶/壳聚糖姜黄素微胶囊制备及其体外人工胃肠道耐受性研究

doi:10.16431/j.cnki.1671-7236.2023.06.015

Abstract

Abstract: 【Objective】 The double-layer coated microcapsules were prepared using pectin and chitosan as wall materials using microencapsulation technology.The optimal preparation conditions were determined by morphological characteristics, mechanical strength and embedding rate analysis, and the artificial gastrointestinal tolerance of the capsules was studied in vitro.【Method】 The first layer of microcapsule coating was composed of 8% pectin, different concentrations of curcumin (3.5, 4.0, 4.5, 5.0 and 5.5 mg/mL) and calcium chloride (CaCl₂) solution (0.3, 0.4, 0.5 and 0.6 mol/L), and curcumin and pectin solution of different proportions (3:7, 2:8 and 1:9 (V/V)).Screening the best preparation conditions of pectin curcumin microcapsules.Using 0.8% chitosan at different pH (4.2, 4.6, 5.0, 5.4 and 5.8) as the second layer of microcapsule coating, then screened the optimum conditions for preparation of pectin/chitosan curcumin microcapsule.Three groups of microcapsules (T1, T2 and T3) were prepared under the optimal screening conditions, and their appearance, mechanical strength, embedding rate, drug loading and gastrointestinal artificial tolerance in vitro were evaluated.【Result】 The results showed that when 8% pectin, 4.5 mg/mL curcumin, the ratio of curcumin to pectin was 1:9, and 0.5 mol/L CaCl₂ as the first layer of microcapsule coating, the embedding rate of pectin curcumin microcapsules was 98%.When 0.8% chitosan at pH 4.6, 5.0 and 5.4 was used as the second layer of microcapsule coating, the microcapsules showed uniform size and regular shape.Three groups of microcapsules were prepared under the optimal screening conditions, among which the mechanical strength, drug loading, protection rate in artificial gastric fluid and release rate in intestinal fluid in group T1 were the highest.【Conclusion】 In this study, with pectin and chitosan as wall materials and curcumin as core materials, the pectin/chitosan curcumin microcapsules were prepared under the optimal screening conditions, which had good morphology, mechanical strength, drug loading and gastrointestinal tolerance in vitro.The results could provide reference for the development of curcumin microcapsules with pectin and chitosan as wall materials.

Key words: curcumin; microcapsules; gastrointestinal tolerance

CLC Number:

S816.79

XU Ziyan, JIANG Yang, LIU Xuetao, LIU Bohan, ZHANG Wen, YAN Changguo, CUI Lianhua. Preparation of Pectin/Chitosan Curcumin Microcapsules and Its Study on Simulated Gastrointestinal Environments[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2312-2320.

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Preparation of Pectin/Chitosan Curcumin Microcapsules and Its Study on Simulated Gastrointestinal Environments

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