果胶/壳聚糖合生元微胶囊制备及其体外人工胃肠道耐受性和储藏性能研究

doi:10.16431/j.cnki.1671-7236.2020.09.012

Abstract

Abstract: Pediococcus acidilactici and inulin were used as wall cores,with pectin and chitosan as the shell material.The conditions of microencapsulation were optimized by analyzing the embedding rate,particle morphology,the mechanical strength,gastrointestinal tolerance in vitro and storage performance.The experimental group was divided into three groups:Free Pediococcus acidilactici group (PA),microcapsule group made by embedding Pediococcus acidilactici alone (PC(PA)MCs),synbiotic microcapsule group made by embedding inulin and Pediococcus acidilactici (PC(PA-I)MCs).The results showed that the appearance,particle size,mechanical strength and embedding rate of microcapsules were better when the mass concentration of pectin was 5 g/100 mL,the content of inulin was 25 mg/mL and the mass concentration of chitosan was 0.8 g/100 mL.Through the gastrointestinal tolerance test in vitro, it was found that PC(PA-I)MCs group was relatively stable in the artificial gastric juice in vitro (P<0.05).At the same time,it began to release rapidly in the artificial intestinal fluid in vitro at 30 min and it was almost completely disintegrated after 2 h.It was found that the cell survival rate of PA group decreased significantly at cold and room temperature through the storage test for 4 weeks.While the cell survival rate of PC(PA-I)MCs group decreased slightly.And the cell survival rate of PC(PA-I)MCs group was higher than that of PC(PA)MCs group (P>0.05).At room temperature,the cell survival rate of PC(PA-I)MCs group was higher than that of PC(PA)MCs group (P>0.05).In summary,pectin/chitosan microcapsules with inulin as probiotics had a better embedding effect on Pediococcus acidilactici.

Key words: Pediococcus acidilacticis; microcapsules; gastrointestinal tolerance; storage stability

CLC Number:

S816.79

CUI Naiyuan, GAO Qingshan, YAN Changguo, CUI Lianhua. Preparation of Pectin/Chitosan Synbiotic Microcapsules and Its Study on Simulated Gastrointestinal Environments and Storage Stability[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2799-2807.

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Preparation of Pectin/Chitosan Synbiotic Microcapsules and Its Study on Simulated Gastrointestinal Environments and Storage Stability

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