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Inhibition of Liposome Transfection siRNA on HDAC1 Expression in Mouse Renal Tubular Epithelial Cells YANG Yang, WU Guo-juan, LI Pei-feng

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Preliminary Study of Different Additives on Beef Cattle Growth and Meat Performance ZHOU Zhen-yong, ZHANG Yang, LIN Hong-kai, ZHANG Jin-shan, LI Hong-bo, YAN Xiang-min, DU Wei

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Research Overview of Milk Urea Nitrogen YANG Lu, XIONG Ben-hai

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The Intestinal Flora Analysis of Four Types of Common Freshwater Fish ZHOU Yu-fa, LI Dai-jun, LIU Dong-yan, LIU Jing-bo, YAO Shu-hong, CAI Yu-mei

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Role of 3' Untranslated Region（3'UTR）in the Regulation of Gene Expression YU Shi-gang, WANG Gang    2016, 43 (11): 2907-2913.   DOI: 10.16431/j.cnki.1671-7236.2016.11.015 Abstract （2023）      PDF（pc） （973KB）（1529）       Knowledge map    Save In eukaryotes,the regulation of gene expression plays an important role in the growth and development of organisms which is a multistep process that involves the transcription,translation and turnover of messenger RNAs（mRNA）and proteins.Researches have showed that the 3'untranslated region（3'UTR）of mRNA is emerging as critically important in regulating gene expression at post transcriptional levels.mRNA stability,localization and translation are largely determined by sequences in the 3'UTR.The aberrant regulation of 3'UTR will result in aberrant levels of expressed protein, enhancing metabolic changes and leading to disease.The researches of 3'UTR in the regulation of gene expression,human diseases and animal production were summarized and discussed in this paper to provide the reference for applications of 3'UTR in human disease diagnosis and treatment and animal performance. Reference | Related Articles | Metrics | Comments（0）

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Establishment of PCR Method for the Detection of Theileria sergenti Based on ITS Gene WANG Yi-nan, JIA Li-jun, XUE Shu-jiang, ZHANG Ying, QIAN Nian-chao, ZHANG Shou-fa

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Xinjiang Strains of Listeria spps of Diverse Origins Serotyped by Multiplex PCR SHANG Wen-jing, ZHAO Xin-fei, WANG Jing-mei, YAN Gen-qiang

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Research Progress on Pharmacokinetics and Residue Elimination of Antibacterial Synergist SUN Ling-ling, YUAN Zong-hui, LI Mei, HUANG Ling-li    2016, 43 (12): 3141-3148.   DOI: 10.16431/j.cnki.1671-7236.2016.12.009 Abstract （239）      PDF（pc） （1111KB）（1495）       Knowledge map    Save Antibacterial synergist can enhance the antibacterial activity of the antibiotics, reduce bacterial resistance and improve treatment effect. Pharmacokinetic study is to develop dosage regimen and evaluate efficacy in clinical.Elimination of residual study can understand drug residual target tissue and residual marker to formulate appropriate withdraw time. This review briefly describes the aditoprim, trimethoprim,diaveridine of three kinds of antibacterial synergist pharmacokinetic and residue elimination rule, emphatically when contrast aditoprim to trimethoprim in the pharmacokinetic parameters of different animals. We can conclude aditoprim with longer elimination half-life and larger apparent volume of distribution, and trimethoprim elimination half-life is different in different animals, diaveridine is often used as intestinal antibacterial synergist. Recently researchers always use high performance liquid chromatography (HPLC) method or chromatographic combination method to study antibacterial synergist on pharmacokinetics and residues elimination research, it shows that the method has good specificity and high sensitivity. Currently due to the irrational use even abuse of antibiotics leading to drug failure and bacterial drug resistance problems, the presence of the antibacterial synergist for solving this problem provide a new direction, and three kinds of antibacterial synergist in different animals pharmacokinetic and residues elimination research is helpful to understand various kinds of drugs commonalities and differences in different animals, in order to find problems of this kind of drug in clinical using and continuously perfect applications of antibacterial synergist in clinical. Reference | Related Articles | Metrics | Comments（0）

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Development and Application of A RT-PCR Method for Detection and Differentiation of Classical and Variant Porcine Reproductive and Respiratory Syndrome Virus WANG Wei-zhong

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Xinjiang Strains of Listeria spps of Diverse Origins Serotyped by Multiplex PCR SHANG Wen-jing, ZHAO Xin-fei, WANG Jing-mei, YAN Gen-qiang

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Risk Analysis of Mercury in Milk: A Review ZHENG Nan, WANG Jia-qi, XU Xiao-min, QU Xue-yin, HAN Rong-wei, HUANG Liang-ce, YE Qiao-yan

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Study on Quick Sex Identification Method in Pigeons by CHD Gene ZHANG Li, SHAN Da-cong, LIU Yan, PAN Yu-hua    2016, 43 (5): 1379-1384.   DOI: 10.16431/j.cnki.1671-7236.2016.05.039 Abstract （392）      PDF（pc） （2103KB）（1451）       Knowledge map    Save In this experiment, the primer pairs were designed, and polymerase chain reaction (PCR) technique to amplify the segments of the partial sex linked gene chromo-helicase-DNA-binding (CHD) to identificate pigeon's sex using feather pulps and blood.We found that two bands were demonstrated in all female egg-laying pigeons, and the sizes of them were 400 and 700 bp, respectively;In all male egg-laying pigeons, only one band appeared with length of 700 bp.100 unknown sex samples were successfully identified (52 males and 48 females).Based on the checking results between molecular electrophoresis of pigeons and the truly sex of maturity pigeons, we detected CHD gene in 2 928 early age pigeons using PCR amplification and electrophoresis in agarose gel.The PCR amplification results showed 1 517 males and 1 411 females. Reference | Related Articles | Metrics | Comments（0）

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Isolation and Identification of the Yellow River Delta Regional Scale Meat Duck Intestines Goods in 2011 LI Feng, LIN Chu-wen, ZHANG Song-lin, MIAO Li-zhong, SHEN Zhi-qiang

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Research and Application of Nano-colloidal Gold Immune Labeling Technique in Bovine Diseases WANG Wu-jun, BAI Quan-yang, ZHENG Teng, XU Shu-fei, KONG Fan-de, HUANG Yi-fan

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Establishment of PCR Method for the Detection of Theileria sergenti Based on ITS Gene WANG Yi-nan, JIA Li-jun, XUE Shu-jiang, ZHANG Ying, QIAN Nian-chao, ZHANG Shou-fa

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Advance on Prediction Methods of B-cell Antigen Epitope MA Fan-shu, ZHANG Lei, WANG Yang, SUN Yan-gang, YAN Xi-jun    2016, 43 (1): 63-67.   DOI: 10.16431/j.cnki.1671-7236.2016.01.010 Abstract （398）      PDF（pc） （988KB）（1394）       Knowledge map    Save Antigen epitopes are the basic functional units of antigen.B-cell antigen epitope prediction is important for vaccine design, development of diagnostic reagents and preparation of high-throughput antibody.This paper summarized the recent advances on prediction methods of B-cell linear epitopes and B-cell conformational epitopes, and compared these prediction methods in order to provide theoretical references for the researches of antigen epitopes. Reference | Related Articles | Metrics | Comments（0）

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Cloning and Sequence Analysis of IL-15 Gene of Chinese KM Mice LI Zhong-yuan, LI Jia-yuan, ZHOU Dong-hui, ZHU Xing-quan, CHEN Jia

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Research Progress on Molecular Etiology and Pathogenesis for African Swine Fever Virus OU Yun-wen, YAN Chuan-zhong, ZHANG Jie, MA Bing, DAI Jun-fei, ZHANG Yong-guang, JIA Ning    2017, 44 (7): 2139-2146.   DOI: 10.16431/j.cnki.1671-7236.2017.07.033 Abstract （444）      PDF（pc） （1048KB）（1381）       Knowledge map    Save African swine fever (ASF) is a devastating disease caused by African swine fever virus (ASFV), characterized by acute feature, high fever, high mortality and other characteristics. ASF mainly outbreaks in African, Eastern Europe countries, Russia and Caucasus region. At present, there are no vaccine available and effective control strategies against ASFV spread, therefore, ASF has a serious impact on the pig industry in the affected countries. The major target cells of the virus are swine reticuloendothelial cells and monocyte-macrophage cells. ASFV can result in apoptosis and affect the host's immune system, and then show the characteristics of the corresponding disease. ASFV has the characteristics of large genome, more genotype and more variability. In this paper, the molecular etiology and pathogenesis of ASFV are reviewed, so as to provide theoretical basis for prevention and control of ASF. Reference | Related Articles | Metrics | Comments（0）

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Sex Identification with Direct Whole Blood PCR Amplification in Chicklings and Chicken Embryos LI Zhi-li, QIN Qing-ming, SHI Zhen-dan, CHEN Hai-nan, HUANG Qun-shan

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Isolation and Identification of Haemophilus parasuis, Cloning and Sequence Analysis of aroA Gene and Institution of Two Program of Triplex PCR Assay MU He-ping, WANG Xing-mei, YE Ya-qiong, LI Jia-wei, LI Yu-gu