马骨髓间充质干细胞向软骨细胞的诱导分化

doi:10.16431/j.cnki.1671-7236.2017.02.017

Abstract

Abstract:

This study was aimed to establish equine bone marrow mesenchymal stem cells(BMSCs) line and induce it to differentiate into chondrocytes. The BMSCs were collected by cutting the bone and flushing the cutting surface by PBS, and then the bone marrow was washed with PBS,the collected cells were cultured after centrifugation. The cells were purified by passaging,the stem cell properties were tested before the induction. And the cells were also appraised by the expression of the special gene of chondrocytes as well as staining the differentiated cells with Alcian blue to insure the induction was effective. The obtained BMSCs expressed Sox2 and Nanog genes, which were the stem cell special genes, and also expressed CD44, CD90 and CD105 genes, but absent of CD34 and CD45 genes. The shapes of the 3rd passage BMSCs were changed after cultured in inducing medium for a few days. Furthermore, the cells were positive to Alcian blue staining, increased expression of the Col special gene of chondrocyte day by day as well. According to this study, the BMSC line was established, and the BMSCs were induced and differentiate into chondrocytes successfully.

Key words: equine; bone marrow mesenchymal stem cells; induced differentiation; chondrocyte

CLC Number:

Q254

LIU Le, WANG Yan-ni, PANG Yu-juan, ZHANG Ting-ting, LIU Zi-jia, ZHU Meng, CAO Jun-wei, ZHANG Yan-ru, LIU Chun-xia. Study on Inducing Equine Bone Marrow Mesenchymal Stem Cells Differentiated into Chondrocytes[J]. , 2017, 44(2): 417-424.

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Study on Inducing Equine Bone Marrow Mesenchymal Stem Cells Differentiated into Chondrocytes

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