端粒逆转录酶基因修饰对绵羊骨髓间充质干细胞端粒酶活性影响的研究

doi:10.16431/j.cnki.1671-7236.2015.06.006

Abstract

Abstract: Bone marrow mesenchymal stem cells (BMSC) had multiple differentiation potentiality,but their life cycle was limited,in order to make further study on the effect of telomerase reverse transcriptase (TERT) gene on activity of telomerase,our research adopted the method of transfecting exogenous TERT into mesenchymal stem cell technology,constructed recombinant expressing vector pcDNA3.1-EGFP-TERT,and then transfecting into ovine BMSCs,and through selection and identification,recombinant expressing vector pcDNA3.1-EGFP-TERT was successfully expressed in BMSCs.Then the TERT-BMSCs were cultured in vitro and the determination of growth curve was conducted,finally,the expression of the original stem cell factor were identified by PCR technique.The results showed that TERT-BMSC had the ability of rapid amplification,cell shape was good and still had the characteristics of stem cells.We got the conclusion that the expression of exogenous TERT gene could activate the activity of BMSCs'telomerase.

Key words: telomerase reverse transcriptase (TERT); vector construction; bone marrow mesenchymal stem cells (BMSC)

CLC Number:

CUI Yan, LI Hao-tian, LIU Zong-zheng, CAO Jun-wei, ZHANG Yan-ru. Study on the Effect of TERT Gene Modification on Activity of Telomerase in Bone Marrow Mesenchymal Stem Cells of Ovis Aries[J]. , 2015, 42(6): 1362-1369.

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Study on the Effect of TERT Gene Modification on Activity of Telomerase in Bone Marrow Mesenchymal Stem Cells of Ovis Aries

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