骨髓间充质干细胞为核供体的克隆绵羊的生产与鉴定

doi:10.16431/j.cnki.1671-7236.2016.11.030

Abstract

Abstract:

In order to obtain the cloned sheep using bone marrow mesenchymal stem cells（BMSCs）as the donor cells,the BMSCs of sheep were chosen and reconstructed embryos were built to transfer.10 published microsatellite markers were chosen,and the DNA samples from clone sheep,donor cells and surrogate ewes were amplified,and the relationship of father-son（RCP）was analyzed using the Quantity One for genotyping.The results showed that the reconstructed embryos were successfully built for electric fusion using sheep BMSCs as nuclear donor,and making nuclear transplantation into enucleated mature oocytes of which the fusion rate was 80.62%.20 surrogate ewe were chosen to be implanted with the reconstructed embryos at morula stage by implant surgery,and 5 lambs were born and only 3 were survived.The genotype of cloned sheep was in line with the dornor cell and the RCP were more than 99.999%.In conclusion,the first clone sheep were obtained successfully by using BMSCs as a nuclear donor in this experiment.

Key words: bone marrow mesenchymal stem cells; somatic cell nuclear transfer; clone sheep

CLC Number:

Q813

GUO Yuan, LIU Zong-zheng, LIU Le, MENG Fan-hua, ZHANG Yan-ru, ZHOU Huan-min, LIU Chun-xia. Production and Identification of Clone Sheep Using Bone Marrow Mesenchymal Stem Cells as Donor Cell[J]. , 2016, 43(11): 3011-3018.

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Production and Identification of Clone Sheep Using Bone Marrow Mesenchymal Stem Cells as Donor Cell

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