绵羊卵丘细胞细胞周期同步化对核移植胚胎发育的影响

doi:10.11843/j.issn.1671-7236.2015.03.023

Abstract

Abstract: The cell cycle synchronization of donor cell is one of the important factors on success of somatic cell nuclear transfer.In this study,we treated the sheep cumulus cells with serum starvation and contact inhibition,then analyzed cell cycle by flow cytometry.The results showed that compared to the control group,24 to 72 h serum starvation increased the proportion of cells at the G₀/G₁ phase (P <0.05).The proportion of cells at the G₀/G₁ phase was increased after contact inhibition 24 to 72 h,but there was no significnant difference between serum starvation and contact inhibition groups (P >0.05),and they were significant higher than control group (P <0.05).Then used these cumulus cells synchronized by serum starvation or contact inhibition as SCNT donor cells,there was no significant difference in the cleavage,morulae and blastocyst rates of two groups (P >0.05),and blastocyst rate of two groups was significant higher than control group (P <0.05).The results suggested that sheep cumulus were effectively synchronized at G₀/G₁ stages by serum starvation or contact inhibition,and could be used as donor cells.

Key words: sheep; cumulus cells; cell cycle synchronization; somatic cell nuclear transfer

CLC Number:

S814.8

SU Wen-long, LI Lu, MENG Na-na, LIU Meng-he, WANG Han-yang, LI Jie-xin, CAO Hui, TIAN Shu-jun, SUN Shu-chun, LI Jun-jie. Effects on Somatic Cell Nuclear Transfer Embryos Development of Cell Cycle Synchronization of Sheep Cumulus Cells[J]. , 2015, 42(3): 658-662.

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Effects on Somatic Cell Nuclear Transfer Embryos Development of Cell Cycle Synchronization of Sheep Cumulus Cells

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