褪黑素受体MT1和MT2在绵羊胃中的分布及表达

doi:10.16431/j.cnki.1671-7236.2022.08.041

Abstract

Abstract: 【Objective】 This study was aimed to detect the distribution and expression of melatonin (MT) specific membrane receptors MT1 and MT2 in different parts of sheep gastric tissues by traditional molecular biological methods to preliminarily explore and clarify the difference of melatonin concentration and action mode in these areas.【Method】 The methods of ELISA, Real-time PCR, immunohistochemistry and Western blotting were used to detect the distribution and expression of melatonin specific membrane receptors MT1 and MT2 in sheep saccus ruminis dorslis, saccus ruminis ventralis, reticulum, omasum and abomasum.【Result】 The results of ELISA showed that melatonin was contained in all gastric tissues of sheep, and the content of melatonin in abomasum was the highest, followed by omasum, and that in saccus ruminis dorslis, saccus ruminis ventralis and reticulum were lower. The results of Real-time PCR showed that the contents of MT1 and MT2 genes mRNA were the highest in the abomasum, followed by saccus ruminis ventralis, and lower in saccus ruminis dorslis and reticulum. Immunohistochemistry results showed that MT1 and MT2 proteins were distributed in all gastric tissues of sheep, mainly expressed in the mucosal layer of each gastric tissue, and the distribution of MT1 and MT2 proteins gradually increased from the bottom to the neck in the abomasum glands. The results of Western blotting showed that the expression of MT1 and MT2 proteins was the highest in reticulum, followed by omasum, and lower in saccus ruminis dorslis, saccus ruminis ventralis and abomasum.【Conclusion】 Melatonin was differentially expressed in various gastric tissues of sheep so as to play a variety of physiological functions. It might regulate the physiological process of rumination after chyme stimulation through the signal transduction system by combining with the specific receptors MT1 and MT2 in gastric tissues.

Key words: sheep; stomach; melatonin; melatonin receptors

CLC Number:

S852.21

LI Weidong, JIANG Yuting, DUAN Hongwei, HE Haijun, YANG Shuai, DING Ziqiang, WU Jianxin, ZHANG Enqiu, HU Junjie. Distribution and Expression of Melatonin Receptors MT1 and MT2 in Various Stomach Tissues of Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3256-3262.

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Distribution and Expression of Melatonin Receptors MT1 and MT2 in Various Stomach Tissues of Sheep

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