提高哺乳动物克隆效率的研究进展

doi:10.16431/j.cnki.1671-7236.2018.09.022

Abstract

Abstract:

Somatic cell nuclear transfer (SCNT),also named as somatic cell cloning technology,has vast importance and implications for biomedicine,genetically modified animals research and livestock breeding or expansion of excellent individuals.Currently,mammalian cloning technology remains inefficient and cloned animals usually accompanies various phenotypic defects.In recent years,researchers have been exploring many ideas,techniques and methods to improve cloning efficiency,which include addition of small molecules in culture media,seeking for superior donor cells like iPS cells,optimization of conditioning parameters for nuclear transfer manipulations and genetic regulation of developmentally important genes such as XIST and H3K9me3 demethylase genes.This paper was aimed to summary above-mentioned progress on cloning efficiency promotion.

Key words: mammalian; somatic cell nuclear transfer (SCNT); cloning efficiency

CLC Number:

S814.8

XU Weihua, WU Zhenfang, SHI Junsong. Research Progress on Methods to Improve Mammalian Cloning Efficiency[J]. , 2018, 45(9): 2516-2523.

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Research Progress on Methods to Improve Mammalian Cloning Efficiency

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