哺乳动物细胞无血清全悬浮培养技术研究进展

doi:10.16431/j.cnki.1671-7236.2021.03.007

Abstract

Abstract: With the rapid development of biological products industry,the serum-free suspension culture technology of mammalian cells has been involved in more and more fields.This technology can expand the scale of mammalian cells,improve the production and quality of biological products,broaden the application field of mammalian cells,and promote industrialized production of mammalian cells.However,compared with the parent cells,the protein expression level and growth regulation pathway of the cells after suspension acclimation have changed to varying degrees,which affected the production performance of cell lines.In this regard,scholars at home and abroad have carried out a lot of research and made good progress.In this paper,the research status of mammalian cells serum-free suspension culture technology and the optimization strategy of clinical production process were descried,in order to provide references for the development of mammalian cells serum-free suspension culture technology.

Key words: mammalian cells; serum-free suspension culture technology; process optimization

CLC Number:

S852.52

WANG Pengpeng, HUANG Shulin, ZHANG Yunke, WANG Zhanhui, WU Wenxue. Research Progress on Serum-free Suspension Culture of Mammalian Cells[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 839-845.

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Research Progress on Serum-free Suspension Culture of Mammalian Cells

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