China Animal Husbandry and Veterinary Medicine ›› 2021, Vol. 48 ›› Issue (3): 839-845.doi: 10.16431/j.cnki.1671-7236.2021.03.007

• Biotechnology • Previous Articles     Next Articles

Research Progress on Serum-free Suspension Culture of Mammalian Cells

WANG Pengpeng1,2, HUANG Shulin3, ZHANG Yunke1,2, WANG Zhanhui1,2, WU Wenxue1,2   

  1. 1. College of Veterinary Medicine, China Agricultural University, Beijing 100193, China;
    2. Lab of Rapid Diagnostic Technology of Animal Diseases, China Agricultural University, Ministry of Education, Beijing 100193, China;
    3. China Animal Husbandry Industry Co., Ltd., Beijing 100070, China
  • Received:2020-07-29 Online:2021-03-20 Published:2021-03-18

Abstract: With the rapid development of biological products industry,the serum-free suspension culture technology of mammalian cells has been involved in more and more fields.This technology can expand the scale of mammalian cells,improve the production and quality of biological products,broaden the application field of mammalian cells,and promote industrialized production of mammalian cells.However,compared with the parent cells,the protein expression level and growth regulation pathway of the cells after suspension acclimation have changed to varying degrees,which affected the production performance of cell lines.In this regard,scholars at home and abroad have carried out a lot of research and made good progress.In this paper,the research status of mammalian cells serum-free suspension culture technology and the optimization strategy of clinical production process were descried,in order to provide references for the development of mammalian cells serum-free suspension culture technology.

Key words: mammalian cells; serum-free suspension culture technology; process optimization

CLC Number: