黄连解毒汤有效成分的提取及其对体内白色念珠菌的清除作用

doi:10.16431/j.cnki.1671-7236.2019.11.032

Abstract

Abstract: The aim of the experiment was to study the extraction of effective fractions of Huanglian Jiedu decoction and its scavenging effect on Candida albicans in vivo.The fractions of Huanglian Jiedu decoction were extracted by water and ethanol reflux,and then the ethanol extract was extracted with three different polar organic solvents (n-butanol,ethyl acetate,petroleum ether).The extraction process was optimized with the minimum inhibitory concentration (MIC) as the index.The changes of fungal load in kidney and liver,and reactive oxygen species (ROS) levels in a mouse model with systemic Candida albicans infection (SC) before or after taking medicine were observed.The levels of cytokines IL-6,IL-1β,pro-IL-1β and TNF-α in serum were measured by ELISA.The results showed that the optimal extraction process of Huanglian Jiedu decoction was the following:The material-liquid ratio was 1:30,the ethanol concentration was 60%,the temperature was 80℃,the extraction time and frequencey were 1 h and 2 times.Under this condition,the MIC₉₀ of EAHD was 0.3125 mg/mL.Compared with the model group,Huanglian Jiedu decoction ethyl acetate extract (EAHD) high dose group significantly reduced the kidney fungal load of mice (P<0.05),and the complete cure rate was 40% which was obvious higher than that of fluconazole group.The fungal load of liver was significantly decreased in EAHD low,medium and high dose groups (50,100 and 200 mg/(kg·d))(P<0.05),and the effect of EAHD medium dose group and fluconazole group on reducing the hepatic fungal load was similar.The ROS content in kidney and liver of EAHD high dose group were significantly or extremely significantly reduced,respectively (P<0.05;P<0.01),and the levels of IL-6,IL-1β and TNF-α in the serum were significantly or extremely significantly increased,respectively (P<0.05;P<0.01).And the pro-IL-1β content in the serum was reduced.In conclusion,the EAHD obtained by the optimal extraction process could reduce ROS content and regulate inflammatory factors in kidney and liver of model mice,thereby alleviate inflammatory reaction to protect tissues and resist Candida albicans.

Key words: extraction process optimization; ethyl acetate extract of Huanglian Jiedu decoction; Candida albicans; fungal load

CLC Number:

R285

YAN Lijun, LYU Xinyu, ZHANG Duo, CHEN Changbin, ZHANG Shuang, GAO Yuan, LUO Jianghan, WANG Fuling, CUI Wenyu, ZHANG Chengxian. Extraction of Effective Fraction of Huanglian Jiedu Decoction and Its Scavenging Effect on Candida albicans in vivo[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(11): 3414-3421.

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Extraction of Effective Fraction of Huanglian Jiedu Decoction and Its Scavenging Effect on Candida albicans in vivo

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