茶多酚对小鼠黑色素瘤细胞B16F10体外增殖和迁移的影响

doi:10.16431/j.cnki.1671-7236.2022.04.009

Abstract

Abstract: 【Objective】 This study was aimed to study the effects of tea polyphenols on the proliferation and migration of mouse melanoma cell B16F10,in order to provide evidence for the use of tea polyphenols in tumor therapy.【Method】 B16F10 cells were treated with different concentrations (0.05,0.10 and 0.15 g/L) of tea polyphenols,no treatment (0 g/L) was used as the control group.CCK-8 was used to determine the cell growth of different treatment groups,and the growth inhibition rate was calculated.After treated with different concentrations of tea polyphenols for 24 h,the migration ability of the cells was evaluated by scratch test.The expression of microphthalmia-associated transcription factor(MITF) and tyrosinase(TYR) mRNA and protein in B16F10 cells were detected by Real-time quantitative PCR and Western blotting.【Result】 Compared with control group,0.05 g/L tea polyphenols had no significant inhibitory effect on the proliferation of B16F10 cells (P>0.05),0.10 and 0.15 g/L tea polyphenols had extremely significant inhibitory effects on B16F10 cells (P<0.01),and the higher the concentration,the stronger the inhibition.0.05,0.10 and 0.15 g/L tea polyphenols had extremely significant inhibitory effects on the migration of B16F10 cells (P<0.01).Real-time quantitative PCR and Western blotting results showed that as the concentration of tea polyphenols increased,the expressions of MITF and TYR mRNA and protein gradually decreased in a concentration-dependent manner.Compared with control group,0.05 g/L tea polyphenols could significantly reduce the expressions of MITF mRNA and protein (P<0.05),0.10 and 0.15 g/L tea polyphenols could extremely significantly decrease the expression of MITF mRNA and protein (P<0.01).0.10 and 0.15 g/L tea polyphenols could extremely significantly decrease the expressions of TYR mRNA and protein (P<0.01).【Conclusion】 0.10 and 0.15 g/L tea polyphenols had significant inhibitory effect on the proliferation and migration of B16F10 cells,and could reduce the expression of melanogenesis genes MITF and TYR,indicating that tea polyphenols could inhibit the progression of melanoma.

Key words: melanoma B16F10 cells; tea polyphenols; proliferation; migration

CLC Number:

R285

LIU Fangjun, LAN Wutao, LI Pengfei, XU Dongmei, ZHANG Lei, ZHU Zhiwei. Effect of Tea Polyphenols on Proliferation and Migration of Mouse Melanoma Cell B16F10 in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1280-1286.

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Effect of Tea Polyphenols on Proliferation and Migration of Mouse Melanoma Cell B16F10 in vitro

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