鸽源性大肠杆菌的分离鉴定及茶多酚对其体外抑菌效果的研究

doi:10.16431/j.cnki.1671-7236.2022.10.038

Abstract

Abstract: 【Objective】 The purpose of this study was to identify the pathogen of diarrhea in a large-scale pigeon farm in Guangzhou and to explore the in vitro bacteriostatic effect of tea polyphenols on the pathogens.【Method】 The feces of sick pigeons in a large-scale pigeon farm in Guangzhou were collected,and the pathogen was identified by isolation and purification,staining and microscopic examination,16S rRNA gene sequencing and phylogenetic tree construction.The drug sensitivity of pathogen was detected by drug sensitivity test,and the virulence genes carried by pathogen were detected by PCR technology.At the same time,the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration(MBC) of tea polyphenols against pathogen and the effects of different concentrations (0 (the control group),125,500,and 1 000 μg/mL) of tea polyphenols on the growth,movement and virulence gene expression of the pathogen were determined by in vitro bacteriostatic test,so as to comprehensively evaluate the in vitro bacteriostatic effect of tea polyphenols on the pathogen.【Result】 From the pathologic materials,a pathogenic strain was isolated and named GG20210604,which biological characteristics were basically consistent with the Escherichia coli.16S rRNA gene sequencing and phylogenetic tree results further confirmed that the strain was Escherichia coli.After detection,GG20210604 was resistant to ciprofloxacin and ampicillin,while carrying a total of four virulence genes,including fliC,fimH,iroN and hlyF.The results of in vitro bacteriostatic test showed,the MIC and MBC values of tea polyphenols against GG20210604 were both 4 000 μg/mL.Compared with the control group,the growth and movement of GG20210604 were significantly inhibited in 250,500 and 1 000 μg/mL of tea polyphenols (P＜0.05);the GG20210604 genes expression of fliC,iroN and hlyF were significantly decreased in 250 and 500 μg/mL of tea polyphenols (P＜0.05),and the genes expression of fimH was also significantly decreased in 250 μg/mL of tea polyphenols (P＜0.05).【Conclusion】 A strain of Escherichia coli was isolated from pigeon.The strain was resistant to ciprofloxacin and ampicillin,while carrying a total of four virulence genes,including fliC,fimH,iroN and hlyF.Tea polyphenols had positive bacteriostatic effect on this strain,which could significantly inhibit its growth,movement and virulence gene expression.This study provided a new idea for preventing and alleviating pigeon diarrhea caused by Escherichia coli,and provided a theoretical basis for the application of tea polyphenols as a plant bacteriostatic agent in pigeon production.

Key words: pigeon; Escherichia coli; isolation and identification; tea polyphenols; bacteriostatic effect

CLC Number:

S858.39

ZHONG Yajing, ZHU Shaoping, CHEN Ailing, MA Tingting, DENG Shan, HUANG Yanhua, FU Jing. Isolation and Identification of Escherichia coli from Pigeon and Study on the Bacteriostatic Effect in vitro of Tea Polyphenols[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 4052-4062.

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Isolation and Identification of Escherichia coli from Pigeon and Study on the Bacteriostatic Effect in vitro of Tea Polyphenols

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