白羽王鸽MyoG基因多态性及其与体尺和肉质性状的关联分析

doi:10.16431/j.cnki.1671-7236.2023.04.018

Abstract

Abstract: 【Objective】 The purpose of this experiment was to study the polymorphism of myogenin (MyoG) gene and its relationship with body size and meat quality traits in White feather King pigeons,and provide reference for the breeding of molecular markers in this breed.【Method】 A total of 108 12-week-old White feather King pigeons were selected as the research objects,PCR and direct sequencing were used to screen the SNP of MyoG gene in White feather King pigeons,RNAfold was used to predict the mRNA secondary structure before and after MyoG gene mutation,and SPSS 26.0 software was used to analyze the correlation between SNP of MyoG gene and body size and meat quality traits of White feather King pigeons.【Result】 Four SNPs were detected in exon 3 of MyoG gene in White feather King pigeons:g.590075 C＞T,g.590084 G＞A,g.590102 G＞A and g.590114 C＞T,all of which were synonymous mutations and resulted in three genotypes,among them,g.590075 C＞T belonged to low polymorphism,the other 3 SNPs showed moderate polymorphism.χ² results showed that g.590084 G＞A and g.590114 C＞T were in Hardy-Weinberg equilibrium (P＞0.05),and there was strong linkage disequilibrium between the two SNPs.The mutations of g.590084 G＞A and g.590102 G＞A resulted in the change of secondary structure and free energy of MyoG gene mRNA sequence.The combination of 4 SNPs produced 4 haplotypes and 9 diplotypes.The results of correlation analysis showed that g.590075 C＞T was extremely significantly associated with keel length in White feather King pigeons (P＜0.01);g.590102 G＞A had an extremely significant effect on body length (P＜0.01).The body length of H3H3 (CCAAAACC) individuals were superior to the other diplotypes,and the keel length and chest width of H4H4 (TTGGGGCC) individuals were superior to the other diplotypes.g.590084 G＞A and g.590114 C＞T were extremely significantly or significantly associated with pectoral muscle shear force (P＜0.01 or P＜0.05).The diplotype H3H3 (CCAAAACC) individuals were better than the other diplotypes in pH and flesh color of chest muscle,while in the shear force and water loss rate,the diplotype H2H4 (CTGAGGCT) and H4H4 (TTGGGGCC) performed better.【Conclusion】 MyoG gene had significant effect on some body size and meat quality traits in White feather King pigeons,which could be used as a candidate gene for breeding new lines in White feather King pigeons.

Key words: White feather King pigeons; MyoG gene; polymorphism; body size trait; meat quality trait; association analysis

CLC Number:

S813.3

TAN Yuancheng, LUO Hualun, ZHANG Yiyu, YANG Suan, WANG Yingtong, WANG Chunyuan. Polymorphism of MyoG Gene and Its Association with Body Size and Meat Traits in White Feather King Pigeons[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1461-1471.

References

[1] MEGENEY L A,RUDINICK M A.Determination versus differentiation and the MyoD family of transcription factors[J].Biochemistry and Cell Biology,1995,73(9-10):723-732.
[2] ZHANG Z T,XU F,ZHANG Y N,et al.Cloning and expression of MyoG gene from Hu sheep and identification of its myogenic specificity[J].Molecular Biology Reports,2014,41(2):1003-1013.
[3] JIUSHENG W,YUEHUAN L,NINGYING X.Histological characteristics of musculus longissimus dorsi and their correlation with restriction fragment length polymorphism of the myogenin gene in Jinghua×Pietrain F(2) crossbred pigs[J].Meat Science,2009,81(1):108-115.
[4] XUE M,ZAN L S,GAO L,et al.A novel polymorphism of the myogenin gene is associated with body measurement traits in native Chinese breeds[J].Genetics and Molecular Research,2011,10(4):2721-2728.
[5] 任思睿.绵羊MyoG和Myf5基因多态性与胴体性状的相关性分析[D].乌鲁木齐:新疆农业大学,2021. REN S R.Correlation analysis of sheep MyoG and Myf5 gene polymorphisms and carcass traits[D].Urumqi:Xinjiang Agricultural University,2021.(in Chinese)
[6] 李蕊清,尹美辰,赵家荣,等.甲基转移酶METTL21C影响鸡骨骼肌细胞成肌的发育[J].基因组学与应用生物学,2019,38(6):2503-2508. LI R Q,YIN M C,ZHAO J R,et al.Effects of METTL21C gene on myogenic development of chicken skeletal muscle cells[J]. Genomics and Applied Biology,2019,38(6):2503-2508.(in Chinese)
[7] 刘宏祥,徐文娟,朱春红,等.鸭胚胎发育中后期胸肌发育阻滞的RNA-Seq分析[J].中国农业科学,2018,51(22):4373-4386. LIU H X,XU W J,ZHU C H,et al.RNA-Seq analysis of on developmental arrest of duck pectoralis muscle during semilate embryonic period[J].Scientia Agricultura Sinica,2018,51(22):4373-4386.(in Chinese)
[8] WEI Y,ZHANG G X,ZHANG T,et al.Myf5 and MyoG gene SNPs associated with Bian chicken growth trait[J].Genetics and Molecular Research,2016,15(3):15037043.
[9] 杨德凤,李维,刘洋,等.MyoG基因多态性与瑶山鸡体尺、屠宰性能及肉质性状的关联分析[J].南方农业学报,2021,52(10):2887-2895. YANG D F,LI W,LIU Y,et al.Correlations between MyoG gene polymorphism and body size,slaughter performance and meat traits of Yaoshan chickens[J].Journal of Southern Agriculture,2021,52(10):2887-2895.(in Chinese)
[10] 唐修君,高玉时,陆俊贤,等.MyoG和A-FABP基因多态性与鹅肉品质性状的相关性研究[J].家畜生态学报,2011,32(6):16-20. TANG X J,GAO Y S,LU J X,et al.Relativity study on the polymorphism of intron 2 of A-FABP and exon 1 of MyoG gene with the meat quality in goose[J].Chinese Journal of Domestic Animal Ecology,2011,32(6):16-20.(in Chinese)
[11] 彭邦星.MSTN、MyoG、MEF2A基因与兴义鸭屠宰性状相关性分析[D].贵阳:贵州大学,2015. PENG B X.Polymorphisms of MyoG,MSTN and MEF2A gene and these relationship with slaughter traits in Xingyi duck[D].Guiyang:Guizhou University,2015.(in Chinese)
[12] 易恒洁,李辉,杨胜林,等.三穗鸭MyoG基因多态性与屠宰性状的相关性研究[J].中国畜牧杂志,2016,52(9):5-10. YI H J,LI H,YANG S L,et al.Polymorphisms of MyoG gene and its relationship with slaughter traits in Sansui ducks[J].Chinese Journal of Animal Science,2016,52(9):5-10.(in Chinese)
[13] 张跟喜,王金玉,唐莹,等.利用MyoG基因培育京海黄鸡快长系的方法[P].江苏:CN104087680B,2015-11-04. ZHANG G X,WANG J Y,TANG Y,et al.Methods for breeding Jinghai Yellow chicken fast growthline with MyoG gene[P].Jiangsu:CN104087680B,2015-11-04.(in Chinese)
[14] 李奕辉,李青青.独龙鸡屠宰性能与肉品质的测定及相关性分析[J].黑龙江畜牧兽医,2021,23:56-61. LI Y H,LI Q Q.Correlation analysis between slaughter performance and meat quality of Dulong chicken[J].Heilongjiang Animal Husbandry and Veterinary Medicine,2021,23:56-61.(in Chinese)
[15] XIONG X,LIU X,ZHU X,et al.A mutation in pHKG1 causes high drip loss and low meat quality in Chinese Ningdu Yellow chickens[J].Poultry Science,2022,101(1):101556.
[16] ZHANG G X,FAN Q C,ZHANG T,et al.Genome-wide association study of growth traits in the Jinghai Yellow chicken[J].Genetics and Molecular Research, 2015,14(4):15331-15338.
[17] ZHANG T,FAN Q C,WANG J Y,et al.Genome-wide association study of meat quality traits in chicken[J].Genetics and Molecular Research, 2015,14(3):10452-10460.
[18] 栾兆进,曲绪仙,贺建宁,等.家畜MyoG基因结构和功能研究进展[J].家畜生态学报,2015,36(7):1-4. LUAN Z J,QU X X,HE J N,et al.Advances on structure and function of myogin livestock animals[J].Chinese Journal of Livestock Ecology,2015,36(7):1-4.(in Chinese)
[19] BAI J Y.Correlation analysis between MyoG gene polymorphism and carcass characteristics of egg quails[J].Indian Journal of Animal Research,2020,54:1223-1228.
[20] 陈哲,陈蓉,闫乐艳,等.鹅MyoG基因启动子活性及转录调控元件分析[J].畜牧兽医学报,2021,52(7):1869-1879. CHEN Z,CHEN R,YAN L Y,et al.Analysis of the promoter activity and transcriptional regulatory elements of goose MyoG gene[J].Acta Veterinaria et Zootechnica Sinica,2021,52(7):1869-1879.(in Chinese)
[21] GENXI Z,YING T,TAO Z,et al.Expression profiles and association analysis with growth traits of the MyoG and Myf5 genes in the Jinghai Yellow chicken[J].Molecular Biology Reports,2014,41(11):7331-7338.
[22] 李丰耘,张茹,王坤,等.盐津乌骨鸡MyoG和IGF-1基因表达及其与生长、屠宰性状的相关性分析[J].中国畜牧兽医,2020,47(8):2502-2509. LI F Y,ZHANG R,WANG K,et al.Expression of MyoG and IGF-1 genes and its correlation analysis with growth and slaughter traits of Yanjin Black-bone chickens[J].China Animal Husbandry & Veterinary Medicine,2020,47(8):2502-2509.(in Chinese)
[23] SERROTE C,REINIGER L,SILVA K B,et al.Determining the polymorphism information content of a molecular marker[J].Gene,2020,726:144175.
[24] WIGGINTON J E,CUTLER D J,ABECASIS G R.A note on exact tests of Hardy-Weinberg equilibrium[J].American Journal of Human Genetics,2005,76(5):887-893.
[25] AKEY J,JIN L,XIONG M.Haplotypes vs single marker linkage disequilibrium tests:What do we gain?[J]. European Journal of Human Genetics,2001,9(4):291-300.
[26] LE BIHAN-DUVAL E,DEBUT M,BERRI C M,et al.Chicken meat quality:Genetic variability and relationship with growth and muscle characteristics[J].BMC Genetics,2008,9:53.
[27] 李云霞,姚新奎,孟军,等.伊犁马MyoG基因外显子1多态性与肉质性状及肌纤维性状关联分析[J].中国畜牧兽医,2021,48(7):2504-2511. LI Y X,YAO X K,MENG J,et al.Correlation analysis between polymorphism of MyoG gene exon 1 with meat quality traits and muscle fiber traits in Yili horse[J].China Animal Husbandry & Veterinary Medicine,2021,48(7):2504-2511.(in Chinese)

Polymorphism of MyoG Gene and Its Association with Body Size and Meat Traits in White Feather King Pigeons

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