苏紫猪SLA-1基因多态性及其抗病潜能分析

doi:10.16431/j.cnki.1671-7236.2023.05.019

Abstract

Abstract: 【Objective】 The purpose of this study was to explore the genetic polymorphism of swine leukocyte antigen-1(SLA-1) and analyze its disease resistance potential,in order to provide a theoretical basis for molecular breeding of Suzi pig.【Method】 The SLA-1 gene was amplified by PCR using blood cell cDNA in Suzi pig as a template and sequenced. Similarity analysis and phylogenetic tree construction were conducted using DNAStar and Mega 5.0 software, respectively.NetMHCpan 4.1 server was used to analyze the ability and characteristics of SLA-1 molecule binding to African swine fever virus (ASFV) antigenic epitopes.The physical and chemical properties,hydrophilic and hydrophobic properties,transmembrane region,structure and function of the encoded proteins were predicted by biological software.【Result】 Six SLA-1 allele sequences of Suzi pig were successfully amplified,and namely SLA-1*sz01,SLA-1*sz02,SLA-1*sz03,SLA-1*sz04,SLA-1*sz05 and SLA-1*sz06.The highly variable sites were mainly located in the α1 and α2 regions of the peptide binding groove (PBG).The similarity comparison results showed that the similarity of the six SLA-1 alleles nucleotide and amino acid sequences of Suzi pig were between 92.9% to 96.9% and 87.0% to 94.8%,respectively.The similarity of the six SLA-1 alleles nucleotide and amino acid sequences of Suzi pig with different pig breeds were between 92.8% to 98.8% and 84.3% to 98.0%,respectively.The prediction results of the binding ability of ASFV derived polypeptides showed that the SLA-1*sz01 protein had a strong ASFV antigen presentation ability,and had highly affinity with B354L protein polypeptide fragment FIDKTTVLY in ASFV.Prediction of functional domain and tertiary structure showed that SLA-1*sz01 was related to the immune system and had a classic SLA-1 tertiary structure.【Conclusion】 In this study,six SLA-1 gene sequences were obtained,among which SLA-1*sz01 could restrict the binding of more ASFV-derived peptides,and had the highest affinity with FIDKTTVLY,which could be used as a candidate gene for disease resistance breeding of Suzi pig.The results provided a reference basis for the research and development of ASFV vaccine.

Key words: Suzi pig; SLA-1 gene; polymorphism; antigen binding site; disease resistance potential

CLC Number:

S813.3

WANG Zeping, WANG Feifei, ZHANG Ruiming, FU Yanfeng, ZHAO Weimin, DAI Chaohui, CHENG Jinhua, LIAO Chao, LI Hui. Analysis on Polymorphism of SLA-1 Gene and Its Disease Resistance Potential in Suzi Pig[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1918-1927.

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Analysis on Polymorphism of SLA-1 Gene and Its Disease Resistance Potential in Suzi Pig

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