F3代波杂山羊VNN1基因多态性及其与产羔数的关联分析

doi:10.16431/j.cnki.1671-7236.2022.04.020

Abstract

Abstract: 【Objective】 This experiment was conducted to investigate the relationship between panthenoyl mercaptoethamine-1 (VNN1) gene polymorphism and lambing number of F3 generation Boza goats,so as to provide molecular guidance for breeding of F3 generation Boza goats.【Method】 107 F3 generation Boza goats were selected for this experiment,blood samples were collected to extract DNA.According to the exon sequence of VNN1 gene(accession No.:NC_030816.1),7 pairs of primers were designed for PCR amplification and sequencing.DNAStar software was used to analyze the sequencing results,and PCR amplification and sequencing were performed on the entire group of possible single nucleotide polymorphism (SNP) sites.Hardy-weinberg equilibrium and genetic diversity analysis were carried out,and the correlation analysis between the different genotypes of VNN1 gene and the lamb number in F3 generation Boza goats was conducted by SPSS 16.0 statistical software.【Result】 There were 7 SNPs in exons 5 and 7 of VNN1 gene (g.10956 A→G,g.11010 C→T,g.11103 C→T,g.11284 C→A,g.11313 T→C,g.18094 C→T and g.18158 G→C),and 3 different genotypes existed in each site.χ² test showed that g.10956 A→G,g.11010 C→T,g.11103 C→T,g.11313 T→C and g.18094 C→T were in Hardy-Weinberg equilibrium.g.11284 C→A and g.18158 G→C were hardy-Weinberg disequilibrium.The polymorphic information content was moderate polymorphis (0.25<PIC<0.5).Correlation analysis showed that g.11010 C→T and g.11313 T→C were significantly correlation with the lamb number in F3 generation Boza goats,CC genotype of g.11010 C→T was lower than CT and TT genotypes;TT genotype of g.11313 T→C was significantly lower than TC and CC genotypes (P<0.05).There was no significant correlation between g.10956 A→G,g.11103 C→T and g.18158 G→C and the lamb number in F3 generation Boza goats (P>0.05).【Conclusion】 There were 7 SNPs in exons 5 and 7 of VNN1 gene,g.11010 C→T and g.11313 T→C of VNN1 gene were significantly correlated with the number of lambs in F3 generation Boza goats,and VNN1 gene could be used as a candidate gene.

Key words: VNN1 gene; F3 generation Boza goats; single nucleotide polymorphism (SNP); lambing number; correlation analysis

CLC Number:

S813.3

RUAN Yong, CHEN Xiang, DAI Lingang, HUANG Jiajin, AN Dongwei, ZOU Qishun. Correlation Analysis of VNN1 Gene Polymorphism and Lambing Number in F3 Generation Boza Goats[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1384-1392.

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Correlation Analysis of VNN1 Gene Polymorphism and Lambing Number in F3 Generation Boza Goats

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