云上黑山羊28号染色体上3个SNPs位点多态性与产羔数性状的关联分析

doi:10.16431/j.cnki.1671-7236.2021.04.016

Abstract

Abstract: In order to verify and discover molecular markers and major genes of lambing traits,541 samples' SNP genotyping of three SNPs (rs268286450,rs268286391 and rs268244272) on chromosome 28 which were selected from Yunshang Black goat in early research was performed by MassARRAY time flight mass spectrometry,and analyzed the relationship between polymorphism of three SNPs and the lambing number in Yunshang Black goat.The results showed that there were a C→T base mutation at 28 498 787 bp of rs268286450,a A→C base mutation at 30 971 277 bp of rs268286391 and a T→C base mutation at 2 707 491 of rs268244272 on chromosome 28.The polymorphism information content (PIC) of three SNPs were 0.36,0.32 and 0.21,respectively.rs268286450 and rs268286391 were in Hardy-Weinberg disequilibrium (P<0.01),while rs268244272 was in Hardy-Weinberg equilibrium (P>0.05).The locus rs268244272 had three genotypes (CC,TT and TC).The wild genotype was TT and its genotype frequency was 0.75.The genotype frequency of TC was 0.22,which was a mutant genotype.The difference of the least squares mean in lambing number between TC and TT genotypes was extremely significant (P<0.01),but not significantly different from CC genotype (P>0.05).There was no significant difference of the least squares mean in lambing number between TT and CC genotypes (P>0.05).The results of association analysis showed that there was no significant correlation between lambing number of Yunshang Black goat and the polymorphism of rs268286450 and rs268286391 (P>0.05),but a significant association existed between the polymorphism of rs268244272 and lambing number (P<0.05).In conclusion,rs268244272 polymorphism on chromosome 28 of Yunshang Black goat was significantly associated with lambing number,and might be used as a molecular marker of lambing number in breeding of high reproduction straits in Yunshang Black goat.

Key words: Yunshang Black goat; SNP locus; chromosome 28; flight mass spectrometry; lambing number; association analysis

CLC Number:

S813.3

ZHU Lan, WANG Peng, HONG Qionghua, YANG Hongyuan, ZHAO Xiaohong, LAN Rong. Association Analysis of Three SNPs on Chromosome 28 with Lambing Number in Yunshang Black Goat[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1294-1301.

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Association Analysis of Three SNPs on Chromosome 28 with Lambing Number in Yunshang Black Goat

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