松辽黑猪OAS1基因多态性及其与繁殖性状的关联分析

doi:10.16431/j.cnki.1671-7236.2021.08.023

Abstract

Abstract: This study was aimed to investigate the association of the polymorphism of oligoadenylate synthase 1 (OAS1) gene with reproductive traits in Songliao Black pigs.130 Songliao Black pigs were selected as the research objects, the SNPs of OAS1 gene exons 1-8 were found by Sanger direct sequencing, and the correlation between SNP of OAS1 gene and reproductive traits of Songliao Black pigs was analyzed using SPSS 19.0 software.The results showed that 33 mutations were detected in exons 2, 3 and 6 of OAS1 gene in Songliao Black pigs.There was a SNP at 110 bp of OAS1 gene exon 2(G110C), which contained three genotypes of GG, GC and CC.There was a SNP at 176 bp of OAS1 gene exon 3(G176T), which contained three genotypes of CC, CT and TT.There was a SNP at 145 bp of OAS1 gene exon 6(C145T), which contained three genotypes of CC, CA and AA.There was a SNP at 166 bp of OAS1 gene exon 6(G166A), which contained three genotypes of GG, GA and AA.There was a SNP site at 206 bp of OAS1 gene exon 6(A206G), which contained three genotypes of AA, AG and GG.The chi-square fitness test showed that G110C of OAS1 gene exon 2 in Songliao Black pigs was in Hardy-Weinberg equilibrium, C176T, C145A, G166A and G206A in exons 3 and 6 deviated from Hardy-Weinberg equilibrium.The results of population genetic parameters analysis showed that the genetic heterozygosity of each SNP was in the middle level andmoderate polymorphism (0.25<PIC<0.5).The correlation analysis results showed that the total number of litter, live litter and weaning piglets of GC genotype in G110C were significantly higher than that of GG genotype (P<0.05).The number of weaned piglets of CT genotype in G176T was significantly higher than that of CC genotype (P<0.05).The total litter number and live number of CC genotype in C145T was significantly higher than that of AA genotype (P<0.05).The number of weaned piglets of GA genotype in G166A was significantly higher than that of GG genotype (P<0.05).The total number of offspring and live litter of GG genotype in A206G were significantly higher than that of AA genotype (P<0.05).The results indicated that there were mutations of OAS1 gene exons, which had significant effect on some reproductive traits in Songliao Black pigs.

Key words: OAS1 gene; Songliao Black pigs; reproductive traits; polymorphism; association analysis; genetic markers

CLC Number:

S813.3

ZHANG Yunpeng, ZHANG Qi, LIU Qingyu, ZHANG Zhibin, ZHANG Qing, ZHANG Jingbo, ZHANG Shumin. Polymorphism of OAS1 Gene and Its Association with Reproductive Traits in Songliao Black Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2910-2919.

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Polymorphism of OAS1 Gene and Its Association with Reproductive Traits in Songliao Black Pigs

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