CTGF、Gprin3基因多态性及其与牦牛体尺性状的关联分析

doi:10.16431/j.cnki.1671-7236.2022.06.022

Abstract

Abstract: 【Objective】 The purpose of this experiment was to detect the single nucleotide polymorphism (SNP) of connective tissue growth factor (CTGF) and G protein-regulated inducer of neurite outgrowth family subtype 3 (Gprin3) genes in yaks, and analyze the association between SNP and body size traits in yaks.【Method】 The SNP of CTGF and Gprin3 genes were detected by gene pool screening technique in 260 individuals of Sibu, Pali, Leiwuqi, Shenzha yaks in Tibet and Maiwa yaks in Sichuan.The polymorphic information content (PIC), number of effective alleles, homozygosity and heterozygosity were analyzed, and the association between SNP and body size traits in yaks was analyzed by least square method (LMS).【Result】 Three SNPs were detected in intron region of CTGF gene in yaks, which were T1537A, C2195T and C2421T.T1537A was in Hardy-Weinberg equilibrium in Sibu and Maiwa yaks, and deviated from Hardy-Weinberg equilibrium in Pali, Leiwuqi and Shenzha yaks, which was significantly associated with body height (P<0.05).C2195T was in Hardy-Weinberg equilibrium and moderately polymorphic in 5 yak populations (0.25<PIC<0.5).C2421T was in Hardy-Weinberg equilibriumin in 5 yak populations, which was significantly associated with the body weight, body length, heart girth, circumference and forelimb length (P<0.05).Four SNPs were identified in Gprin3 gene, which were G310C, C414T, C1100T and G1213A.G310C, C414T and C1100T were in Hardy-Weinberg equilibrium in 5 yak populations, G1213A deviated from Hardy-Weinberg equilibrium in Leiwuqi yaks, and accorded with Hardy-Weinberg equilibrium in other 4 yak populations.G310C was low polymorphic in 5 yak populations (PIC<0.25).C414T and C1100T were moderately polymorphic in Leiwuqi and Sibu yaks, G1213A was moderately polymorphic in Leiwuqi yaks, but was low polymorphic in other populations.C414T was significantly associated with the body weight, body height, body length and heart girth in 5 yak populations, C1100T was significantly associated with the circumference and forelimb length in Sibu and Shenzha yaks (P<0.05).【Conclusion】 CTGF and Gprin3 genes had abundant polymorphism, T1537A and C2421T of CTGF gene, C414T and C1100T of Gprin3 gene, were associated with body size traits in yaks, which could be used as candidate genes and loci affecting the growth and development traits for directional breeding of yaks.

Key words: yaks; CTGF gene; Gprin3 gene; polymorphism; body size traits

CLC Number:

S823

CHAI Zhixin, WU Zhijuan, WANG Jikun, ZHANG Chengfu, ZHONG Jincheng, XIN Jinwei. Polymorphisms of CTGF and Gprin3 Genes and Their Association with Body Size Traits in Yaks[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2216-2227.

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Polymorphisms of CTGF and Gprin3 Genes and Their Association with Body Size Traits in Yaks

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