儋州鸡体尺性状全基因组关联分析

doi:10.16431/j.cnki.1671-7236.2022.02.021

Abstract

Abstract: 【Objective】 This experiment was aimed to explore the effective SNP and functional genes related to body size traits in chickens, and provide effective theoretical support for Danzhou chickens.【Method】 Blood samples of 200 Danzhou chickens were collected and used to extract genomic DNA.Genome-wide SNP markers were obtained by using a depth of 10× whole genome resequencing, and analyzed the genotypes of individuals.Genome-wide association study (GWAS) was performed using a mixed-effects model and linear regression approach for body size traits (shank length, shank circumference, body slope length, breast width, pelvis width, breast depth, and fossil bone length) of 70-day-old Danzhou chickens by EMMAX.【Result】 The results showed that 12 and 8 SNPs were significantly related to the shank length and the shank circumference.SNPs associated with shank length traits were located on chromosomes 1, 2, 4 and 8.SNPs associated with shank circumference traits were located on chromosomes 2, 4, 8 and 13.The experiment predicted that the candidate genes associated with shank length were KCNA1, TPK1, EZH2, FSTL5 and AMY2A, and the candidate genes associated with shank circumference were TPK1, FSTL5, AMY2A, TGFBI, LECT2 and IL-9.Through KEGG pathway analysis and GO annotation, it was found that 8 genes were involved in biological processes such as potassium ion transmembrane transport, thiamine metabolism, cell proliferation, calcium binding, skeletal muscle satellite cell maintenance and skeletal muscle regeneration, cell receptor interaction, growth factor activity and so on.【Conclusion】 In this experiment, 20 SNPs associated with body size traits were found, and 8 candidate genes for the target traits were screened, which provided candidate molecular markers for Danzhou chickens breeding and new ideas for marker assisted selection of local.

Key words: Danzhou chickens; genome wide association study (GWAS); SNP; body size traits

CLC Number:

S831.2

JIANG Hongzheng, YANG Dezhi, MA Zhonghua, XUN Wenjuan, SHI Liguang, HOU Guanyu. Genome-wide Association Study of Body Size Traits in Danzhou Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 598-607.

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Genome-wide Association Study of Body Size Traits in Danzhou Chickens

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