梅花鹿MSRB3基因多态性及其与茸重性状的关联分析

doi:10.16431/j.cnki.1671-7236.2022.11.018

Abstract

Abstract: 【Objective】 This study aimed to explore the polymorphism of methionine sulfoxide reductase B3 (MSRB3) gene and its association with antler weight trait.【Method】 8 sika deer with high and 8 sika deer with low antler yield were selected, and the gene mutation sites were detected by direct DNA sequencing. The genetic variation of MSRB3 gene in 314 24-month-old sika deer was genotyped by MassARRAY^® SNP typing technology, and the association analysis and haplotype analysis were carried out combined with the data of antler weight traits of sika deer.【Result】 A total of 6 SNPs were found in MSRB3 gene of sika deer, of which 2 SNPs were located in the exon region, and the mutations did not cause amino acid changes, belonging to synonymous mutations, and the other 4 SNPs were located in the intron region.The typing results showed that 4 samples were not successfully typed, and the remaining 310 samples were subjected to follow-up analysis.The observed heterozygosity of each locus was basically consistent with the expected heterozygosity.The heterozygosity of g.44455582 T>C, g.44455759 C>T, g.44414424 T>C, g.44350306 T>C and g.44340836 G>A were higher, which showed moderately polymorphic (0.25<PIC<0.5).And g.44340734 G>C locus had low heterozygosity, which showed low polymorphism (PIC<0.25).The results of chi-square test showed that g.44455759 C>T deviated from Hardy-Weinberg equilibrium (P<0.05), and the other 5 loci were in Hardy-Weinberg equilibrium (P>0.05).The results of association analysis between the different genotypes of 6 SNPs and antler weight of sika deer showed that the antler weight of TT genotype with g.44455582 T>C locus was extremely significantly higher than that of CC and TC genotypes (P<0.01).The haplotype analysis results showed that g.44340734 G>C and g.44350306 T>C, g.44455582 T>C and g.44455759 C>T were strongly linked, each producing 3 haplotypes.The antler weight of haplotype TC in Block 2 was significantly higher than that of haplotype CT (P<0.05).【Conclusion】 MSRB3 gene was closely related to the antler weight traits of sika deer, g.44455582 T>C locus and haplotype TC could be used as molecular markers for screening high-yield sika deer.

Key words: sika deer; MSRB3 gene; SNPs; antler weight

CLC Number:

S825

ZHOU Ya, ZHANG Heyang, LIU Linling, LI Haodong, ZHENG Junjun, WANG Guiwu. Polymorphism of MSRB3 Gene and Its Association Analysis with Antler Weight Traits in Sika Deer[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4279-4287.

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Polymorphism of MSRB3 Gene and Its Association Analysis with Antler Weight Traits in Sika Deer

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