哈萨克羊iNOS基因多态性与布鲁氏菌病的相关性分析

doi:10.16431/j.cnki.1671-7236.2021.03.035

Abstract

Abstract: The purpose of this experiment was to explore the correlation between polymorphism of inducible nitric oxide synthase (iNOS) gene and brucellosis in Kazakh sheep.The serum of 231 Kazakh sheep were tested for serology by brucellosis using RBPT method.Refer to the sheep iNOS gene sequence in GenBank,design primers for its exons 6,7,8,PCR-SSCP and DNA sequencing technology were used to detect the polymorphism of iNOS gene in 231 Kazakh sheep,and analyze the correlation between their SNPs and the susceptibility to brucellosis.The detection results showed that 67 Kazakh sheep were positive for Brucella infection,and the positive detection rate was 29.00%.No polymorphic sites were detected on the exons 6 and 8 fragments of iNOS gene in Kazakh sheep,F7-T18054C and F7-C18084T were detected on the exon 7 fragment,and three genotypes (TC,TT and CC) were detected on the F7-T18054C polymorphic site,the dominant allele and genotype were C and CT genotype,and the allele frequency and genotype frequency were 0.660 and 0.446,respectively.Two genotypes (CT and CC) were detected on the F7-C18084T polymorphic site.The dominant allele and genotype were C and CC,the allele frequency and genotype frequency were 0.946 and 0.892,respectively.F7-C18084T belonged to low polymorphism (PIC<0.25),F7-T18054C belonged to moderate polymorphism (0.25 < PIC < 0.5).The correlation analysis results showed that the correlation between F7-T18054C and F7-C18084T polymorphic sites and brucellosis susceptibility was not significant (P>0.05).The results showed that F7-T18054C and F7-C18084T of iNOS in Kazakh sheep didn't correlate with the susceptibility to brucellosis.

Key words: iNOS gene; Kazakh sheep; PCR-SSCP; SNPs; brucellosis

CLC Number:

S855.1⁺2

ZHANG Fan, XU Jie, LI Gang, ZHOU Yongshun, HE Ying, HAN Kai, CHEN Fulong, GAO Jianfeng. Correlation Analysis of iNOS Gene Polymorphism and Brucellosis in Kazakh Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(3): 1102-1111.

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Correlation Analysis of iNOS Gene Polymorphism and Brucellosis in Kazakh Sheep

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