牦牛乳铁蛋白基因多态性及其与乳品质性状的关联分析

doi:10.16431/j.cnki.1671-7236.2021.05.016

Abstract

Abstract: In this study,polymorphisms of yak lactoferrin (LF) gene were investigated and their effects on some milk quality traits were estimated so as to enrich the molecular genetic data for yak.Variations in 5'UTR and intron 4 of LF gene were checked by PCR-SSCP in 3 yak populations (Gannan yak,Tianzhu White yak and Tibetan yak) and their effects on the milk quality traits were analyzed in Gannan yak.The results showed that:Variation of c.-568 G>A and c.499+56 C>A were found in 5'UTR and intron 4 of yak LF gene,respectively.Allele A₁,genotype A₁B₁ of 5'UTR and genotype A₂B₂ of intron 4 were the dominant allele and genotype in three yaks.The allele A₂ of intron 4 was dominant in Gannan yak and Tianzhu White yak,but allele B₂ was dominant in Tibetan yak.The polymorphism information content (PIC) in 5'UTR and intron 4 of LF gene in yaks ranged from 0.25 to 0.50,belonging to moderate polymorphism.The association analysis showed that the variations in LF gene affected the milk quality traits in different parities of Gannan yak.Individuals with genotypes A₁A₁ and A₁B₁ of 5'UTR in second parity yak had significantly higher non-fat solids rate than B₁B₁ genotype (P<0.05),the genotypes A₂B₂ and B₂B₂ of intron 4 in the third parity had significantly higher milk fat rate than A₂A₂ genotype (P<0.05),as well as yaks with B₂B₂ genotype in fourth parity had significant lower milk rate than A₂A₂ and A₂B₂ genotype (P<0.05).In conclusion,the polymorphisms were found in LF gene of Gannan yak,Tianzhu White yak and Tibetan yak,and these variations in 5'UTR and intron 4 that were association with content of non-fat solids and milk fat rate of Gannan yak,respectively,could be used as potential molecular genetic markers for milk quality traits.

Key words: yak; LF gene; PCR-SSCP; milk quality traits

CLC Number:

S823.8⁺5

YANG Xiaobin, HE Lili, ZHANG Yang, ZHOU Ruifeng, HOU Kangli, ZHANG Yunyun, WANG Yueli, LI Qingqing, SHI Bingang, HU Jiang. Polymorphisms of LF Gene and Their Association with Milk Quality Traits in Yak[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(5): 1655-1663.

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Polymorphisms of LF Gene and Their Association with Milk Quality Traits in Yak

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