China Animal Husbandry and Veterinary Medicine ›› 2021, Vol. 48 ›› Issue (9): 3293-3302.doi: 10.16431/j.cnki.1671-7236.2021.09.020

• Animal Nutrition and Feed Science • Previous Articles     Next Articles

Bacterial Flora Composition of Normal and Diarrhea Yak Calves Based on 16S rRNA Sequencing

ZHANG Yuying1,2,3, LIU Shujie1,2,3, FENG Yuzhe1,2,3, ZHANG Xiaowei1,2,3, CUI Zhanhong1,2,3   

  1. 1. College of Animal Science and Veterinary Sciences, Qinghai University, Xining 810016, China;
    2. Qinghai Yak Engineering Research Center, Xining 810016, China;
    3. Qinghai Provincial Key Laboratory of Animal Nutrition and Feed Science for Plateau Grazing Livestock, Xining 810016, China
  • Received:2021-04-02 Online:2021-09-20 Published:2021-09-17

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Abstract: This experiment was conducted to compare and analyze the fecal microflora composition and gene function prediction of normal and diarrhea yak calves under grazing conditions, and screen out the bacteria as the basis for diarrhea evaluation, so as to provide the theoretical basis for the development of probiotics for yak calves in the next step. Seven fecal samples were collected from normal and diarrhea yak calves in Yushu area of Qinghai province under grazing conditions, respectively, and DNA was extracted. 16S rRNA sequencing technology was used to study the OTU clustering, relative abundance difference at phylum level and genus level, Alpha diversity, Beta diversity and functional prediction level information of intestinal microflora of yak calves between normal and diarrhea groups. The results showed that at phylum level, Bacteroidetes and Firmicutes were the dominant phyla in normal and diarrhea groups, and the Fusobacterium in diarrhea group was significantly higher than that in normal group (P<0.05). At genus level, the relative abundance of dominant bacteria in the feces of normal group calves from high to low were Bacteroides, Alloprevotella and unidentified_Ruminococcaceae. The relative abundance of dominant bacteria in the feces of diarrhea group calves from high to low were Bacteroides, Fusobacterium, unidentified_Enterobacteriaceae and unidentified_Ruminococcaceae, and the abundance of Fusobacterium in diarrhea group was significantly higher than that in normal group (P<0.05). At function prediction level, the main contents of glycan biosynthesis and metabolism, transportation and catabolism, aging, classification and degradation and lipid metabolism in diarrhea group were significantly higher than that in normal group (P<0.05), while the main contents of amino acid metabolism, signal transduction, cell movement and genetic information in normal group were significantly higher than that in diarrhea group (P<0.05). According to this experiment, the difference of dominant flora between normal and diarrhea groups of grazing yak calves was analyzed, the diarrhea of calves in this experiment was caused by Fusobacterium.

Key words: yak calves; diarrhea; fecal; bacterial flora; 16S rRNA sequencing

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