Abstract: Bovine viral diarrhea(BVD) is an infectious disease caused by Bovine viral diarrhea virus (BVDV) infection.BVD will lead to the decrease of animal performance and economic losses in global cattle industry.The clinical symptoms and pathological changes of BVD are similar to other bovine-derived diarrhea virus diseases.Therefore,it is necessary to select a fast and efficient BVDV diagnosis method according to different conditions,then take immediate measures to block BVDV transmission between cattle,between PI cattle and healthy cattle,so as to reduce the economic losses caused by BVDV to the cattle industry.There are many methods for BVDV detection,mainly include virus isolation and identification,Real-time quantitative RT-PCR,RT-PCR,loop-mediated isothermal amplification (LAMP),recombinase polymerase amplification (RPA),colloidal gold immunodetection technology (GICT),enzyme-linked immunosorbent assay (ELISA),antigen capture ELISA (AC-ELISA),indirect immunofluorescence (IFA),etc.In recent years,some new technologies and optimal methods have been applied to the detection of BVDV,such as antibody liquid chip technology,CRISPR-LwCas13a,dual nano RT-PCR method,all of which have the advantages of strong specificity and high sensitivity.The author summarizes research progress of BVDV detection methods in two aspects of antigen detection and antibody detection,and compares the advantages and disadvantages of each detection method.It will be convenient for veterinary research institutions and departments at all levels to select appropriate and efficient BVDV detection methods according to the actual situation,so as to provide a reference for the epidemiological investigation and prevention of BVD in China.

Key words: bovine viral diarrhea; antigen detection; antibody detection