小分子药物索非布韦对牛病毒性腹泻病毒体外复制的影响

doi:10.16431/j.cnki.1671-7236.2021.07.042

Abstract

Abstract: The aim of this study was to investigate whether Sofosbuvir,a small molecule drug,could inhibit the replication of Bovine viral diarrhea virus (BVDV).The effects of Sofosbuvir on the proliferation of bovine kidney cells (MDBK) at different time and concentrations were determined by MTT method.The optimal concentration of Sofosbuvir which could effectively inhibit BVDV replication was screened by immunofluorescence staining test.The effects of Sofosbuvir on BVDV replication were detected by Real-time PCR,CPE and TCID₅₀.The results showed that Sofosbuvir treated MDBK cells for 24 and 48 h extremely significantly inhibited the proliferation of cells (P<0.01).When Sofosbuvir was 800 μmol/L,the content of double stranded RNA (dsRNA) in MDBK cells infected with BVDV was extremely significantly lower than that in the control group (P<0.01).Real-time PCR showed that compared with DMSO treatment group,the expression of BVDV 5'UTR mRNA in Sofosbuvir treated MDBK cells was extremely significantly decreased at 24 and 48 h after infection (P<0.01).The pathological changes of MDBK cells infected with Sofosbuvir were significantly reduced.Sofosbuvir treatment for 24 h extremely significantly reduced the formation and release of virus particles in the offspring of MDBK cells infected with BVDV (P<0.01).In conclusion,Sofosbuvir could effectively inhibit BVDV replication in vitro.

Key words: Sofosbuvir; Bovine viral diarrhea virus (BVDV); anti-viral drug

CLC Number:

S852.65

FU Qiang, HE Yuanxiu, LI Zeyu, JIEENSI Sailike, ZHOU Yuxin, YANG Li, RAN Duoliang, SHI Huijun. Effects of Small Molecular Drug Sofosbuvir on Bovine Viral Diarrhea Virus Replication in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2653-2660.

References

[1] LANYON S,HILL F,REICHEL M,et al.Bovine viral diarrhoea:Pathogenesis and diagnosis[J].Veterinary Journal,2014,199(2):201-209.
[2] MACHADO G,EGOCHEAGA R,HEIN H,et al.Bovine viral diarrhoea virus (BVDV) in dairy cattle:A matched case control study[J].Transboundary and Emerging Diseases,2016:63(1):e1-13.
[3] SUDA Y,MURAKAMI S,HORIMITO T.Bovine viral diarrhea virus non-structural protein NS4B induces autophagosomes in bovine kidney cells[J].Archives of Virology,2019,164(1):255-260.
[4] 李新培,周伟光,关平原,等.牛病毒性腹泻病毒致病机制研究进展[J].中国畜牧兽医,2018,45(8):2303-2311. LI X P,ZHOU W G,GUAN P Y,et al.Research progress on pathogenesis of Bovine viral diarrhoea virus[J].China Animal Husbandry & Veterinary Medicine,2018,45(8):2303-2311.(in Chinese)
[5] APPLEGATE T,FAJARDO E,SACKS J.Hepatitis C virus diagnosis and the holy grail[J].Infectious Disease Clinics of North America,2018,32(2):425-445.
[6] 周红潮,王慧,张旭,等.药物抗牛病毒性腹泻病毒的研究进展[J].黑龙江畜牧兽医,2019,9:34-38. ZHOU H C,WANG H,ZHANG X,et al.Research progress on drugs against BVDV[J].Heilongjiang Animal Science and Veterinary Medicine,2019,9:34-38.(in Chinese)
[7] LAWITZ E,ALESSAND M.Sofosbuvir for previously untreated chronic Hepatitis C infection reply[J].Global Public Health,2013,8(5):678-679.
[8] WELZEL T,NELSON D,MORELLI G,et al.Effectiveness and safety of sofosbuvir plus ribavirin for the treatment of HCV genotype 2 infection:Results of the real-world,clinical practice HCV-TARGET study[J].Gut,2017,66(10):1844-1852.
[9] TAGNOUOKAM N P,NGOUFACK M,KENMOE S,et al.Hepatitis C virus genotyping based on core and NS5B regions in cameroonian patients[J].Virology Journal,2019,16(1):101-108.
[10] 梁霄勇,季新成,冉多良.牛病毒性腹泻病毒TC株的分离鉴定及E0基因的序列分析[J].畜牧与兽医,2016,48(7):36-40. LIANG X Y,JI X C,RAN D L.Isolation and identification of Bovine viral diarrhea virus TC strain and sequence analysis of E0 gene[J].Animal Husbandry & Veterinary Medicine,2016,48(7):36-40.(in Chinese)
[11] 田瑞鑫,李胜男,胡新艳,等.牛miR-29b影响牛病毒性腹泻病毒感染BALB/c小鼠的作用[J].中国兽医学报,2020,40(2):264-271. TIAN R X,LI S N,HU X Y,et al.Effect of bovine miRNA-29b on Bovine viral diarrhea virus infection in BALB/c mice[J].Chinese Journal of Veterinary Science,2020,40(2):264-271.(in Chinese)
[12] TAUTZ N,TEWS B,MEYERS G.The molecular biology of Pestiviruses[J].Advances in Virus Research,2015,93(3):47-160.
[13] WALZE P,RIDDELL K,NEWCOMER B,et al.Comparison of reproductive protection against Bovine viral diarrhea virus provided by multivalent viral vaccines containing inactivated fractions of Bovine viral diarrhea virus 1 and 2[J].Vaccine,2018,36(26):3853-3860.
[14] FUKUHARA T,MATSUURA Y.Roles of secretory glycoproteins in particle formation of Flaviviridae viruses[J].Microbiology and Immunology,2019,63(10):401-406.
[15] JACKSON W,EVERSON G.Sofosbuvir and velpatasvir for the treatment of hepatitis C[J].Expert Review of Gastroenterology & Hepatology,2017,11(6):501-505.
[16] 刘占悝,李智杰,刘泽余,等.中药金银花体外抗牛病毒性腹泻病毒的作用研究[J].黑龙江畜牧兽医,2020,14:116-120. LIU Z L,LI Z J,LIU Z Y,et al.Study on the effect of Chinese herbal medicine honeysuckle anti-Bovine viral diarrhea virus in vitro[J].Heilongjiang Animal Science and Veterinary Medicine,2020,14:116-120.(in Chinese)
[17] TONELLI M,SIMONE M,TASSO B,et al.Antiviral activity of benzimidazole derivatives.Ⅱ.Antiviral activity of 2-phenylbenzimidazole derivatives[J].Bioorganic & Medicinal Chemistry,2010,18(8):2937-2953.
[18] 郭振环,马霞,王恬,等.蜂胶黄酮对宿主细胞抗病毒能力的影响[J].中国畜牧兽医,2016,43(8):2190-2195. GUO Z H,MA X,WANG T,et al.Effects of propolis flavone on antiviral ability of host cell[J].China Animal Husbandry & Veterinary Medicine,2016,43(8):2190-2195.(in Chinese)
[19] RIEDEL C,LAMP B,CHEN H,et al.Fluorophore labelled BVDV:A novel tool for the analysis of infection dynamics[J].Scientific Reports,2019,9(1):5972-5973.
[20] RAJPUT M K S,ABDELSALAM K,DARWEESH M F,et al.Both cytopathic and non-cytopathic Bovine viral diarrhea virus (BVDV) induced autophagy at a similar rate[J].Veterinary Immunology and Immuno-pathology,2017,193(1):193-194..
[21] GOMEZ R N,BASURTO A F,VERDUGO R A,et al.Genetic diversity of Bovine viral diarrhea virus in cattle from Mexico[J].Journal of Veterinary Diagnostic Investigation,2017,29(3):362-365.
[22] MUSHEN M,LEE P.Quantitative and rapid interference of Bovine viral diarrhea virus BVDV/END^- and BVDV/END⁺ strains[J].Acta Virologica,2018,62(3):333-336
[23] 周淑棉,曹迷霞,胡庭俊.辣蓼黄酮正丁醇部分体外抗猪繁殖与呼吸综合征病毒的研究[J].中国畜牧兽医,2020,47(2):605-611. ZHOU S M,CAO M X,HU T J.Study on effect of anti-PRRSV of n-butanol part of flavonoids from Polygonum hydropiper L.in vitro[J].China Animal Husbandry & Veterinary Medicine,2020,47(2):605-611.(in Chinese)

Effects of Small Molecular Drug Sofosbuvir on Bovine Viral Diarrhea Virus Replication in vitro

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LIU Kexin, WANG Chao, ZHANG Ao, LIU Jiali, TAN Bin, ZHANG Shuqin. Isolation,Identification and Genome Sequence Analysis of Bovine Viral Diarrhea Virus LN-1 Strain [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1150-1159.
[2]	SHAO Baihui, JIANG Dongjun, XIE Yimeng, LIU Siyu, SHAO Ziyi, ZHANG Zecai, JI Hong, ZHU Zhanbo. Molecular Docking Prediction of Artemisinin and NS5B Protein of Bovine Viral Diarrhea Virus and Its Antiviral Effect [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3581-3588.
[3]	WANG Wei, HAN Huihui, DING Naizheng, HE Chengqiang. Prokaryotic Expression of Bovine Viral Diarrhea Virus E0 Protein and Preparation of Polyclonal Antibody [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3131-3139.
[4]	REN Jie, LIN Ling, TANG Deyuan, ZENG Zhiyong, WANG Bin, YU Guangmei, ZHENG Ruwen, WU Daoyi, HUANG Tao. Construction of Bovine Viral Diarrhea Virus E0-E2 Gene Fusion Adenovirus and Evaluation of Immune Effect in Mice [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2056-2063.
[5]	MA Baoyi, SHENG Chenyan, LIU Hongying, MA Qingxia, WANG Tingting, LI Jianming, SHI Kun, DU Rui, LENG Xue. Establishment of a Duplex TaqMan Real-time PCR Method for Detection of Bovine Viral Diarrhea Virus Types 1 and 2 [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2326-2335.
[6]	WU Tongzhong, NUERSAILIKE Nusufu, HUANG Xin, HAN Mengli, ZHANG Xingxing, ZHANG Qian, WANG Xinhua, HE Yanhua, ZHONG Fagang. Construction of Recombinant Lactococcus Expressing E2 Protein of Bovine Viral Diarrhea Virus and Immunogenicity Analysis of the Protein [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2975-2981.
[7]	CHANG Liyun, LIU Zhiyong, QIN Jianhua, ZHAO Yuelan. Epidemiological Investigation of Viral Diarrhea of Dairy Calves in Tangshan City, Hebei Province [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(11): 4213-4219.
[8]	YANG Yan, QIAN Tianhao, ZHANG Yanhong, ZHANG Zhe, HAO Xiujing, LI Yan, LYU Wenhua, LIU Zhao, SHENG Jinliang. Screening and Reactogenicity Test of Bovine Viral Diarrhea Virus NS3 Protein Nanobody [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(1): 303-311.
[9]	ZHANG Chao, HE Jinke, HE Yanhua, MA Xusheng, CHEN Chuangfu. Isolation of Bovine Viral Diarrhea Virus Type 2 in Fetal Bovine Serum and Detection of Antibodies in Skimmed Milk Powder [J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2239-2247.
[10]	ZHANG Shuqin, TAN Bin, LIU Zeyu, WANG Chao, GUO Li, SUN Na, CHENG Shipeng. Isolation,Identification and Pathogenicity Analysis of Bovine Viral Diarrhea Virus JLS-01 Strain from Porcine [J]. , 2019, 46(2): 557-564.
[11]	HE Jinke, YANG Yajun, DENG Xiaoyu, HE Yanhua, ZHANG Chao, MA Zhongchen, WANG Yong, CHEN Chuangfu. Bioinformatics Analysis and Multi-epitope Screening of NS3 Protein of Bovine Viral Diarrhea Virus [J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3475-3485.
[12]	LI Xinpei, ZHOU Weiguang, GUAN Pingyuan, CHENG Shipeng, WEN Yongjun. Research Progress on Pathogenesis of Bovine Viral Diarrhoea Virus [J]. , 2018, 45(8): 2303-2311.
[13]	WANG Danyang, WANG Xurong, ZHANG Kang, WANG Hairui, WANG Lei, ZHANG Jingyan, ZHANG Kai, LI Jianxi, WANG Xuezhi. Study on Calves Diarrhea Caused by Mixed Infection of Bovine Viral Diarrhea Virus, Escherichia coli and Proteus mirabilis [J]. , 2018, 45(1): 189-195.
[14]	WANG Mu-chuan, YUE Hua, TANG Cheng, YANG Ze-lin. Detection and Genetic Evolution of Diarrhea-related Viruses in Chongqing Beef Cattle [J]. , 2017, 44(9): 2731-2738.
[15]	CHEN Xin-nuo, ZHANG Bin. Research Progress on Molecular Biology of Bovine Viral Diarrhea Virus [J]. , 2017, 44(11): 3137-3142.